ddCt

The ddCt Algorithm for the Analysis of Quantitative Real-Time PCR (qRT-PCR)

Bioconductor version: 2.6

The Delta-Delta-Ct (ddCt) Algorithm is an approximation method to determine relative gene expression with quantitative real-time PCR (qRT-PCR) experiments. Compared to other approaches, it requires no standard curve for each primer-target pair, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The ddCt package implements a pipeline to collect, analyse and visualize qRT-PCR results, for example those from TaqMan SDM software, mainly using the ddCt method. The pipeline can be either invoked by a script in command-line or through the API consisting of S4-Classes, methods and functions.

Author: Rudolf Biczok, Markus Ruschhaupt and Jitao David Zhang

Maintainer: Jitao David Zhang <j.zhang at dkfz.de>

To install this package, start R and enter:

    source("http://bioconductor.org/biocLite.R")
    biocLite("ddCt")

To cite this package in a publication, start R and enter:

    citation("ddCt")

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