crisprVerse: ecosystem of R packages for CRISPR gRNA design

Installation and getting started

The crisprVerse is a collection of packages for CRISPR guide RNA (gRNA) design that can easily be installed with the crisprVerse package. This provides a convenient way of downloading and installing all crisprVerse packages with a single R command.

The package can be installed from the Bioconductor devel branch using the following commands in an R session:

if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install(version="devel")
BiocManager::install("crisprVerse")

The core crisprVerse includes the packages that are commonly used for gRNA design, and are attached when you attach the crisprVerse package:

library(crisprVerse)

You can check that all crisprVerse packages are up-to-date with the function crisprVerse_update().

Components

The following packages are installed and loaded with the crisprVerse package:

Reproducibility

sessionInfo()
## R version 4.5.2 (2025-10-31)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 24.04.3 LTS
## 
## Matrix products: default
## BLAS:   /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3 
## LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.26.so;  LAPACK version 3.12.0
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_US.UTF-8        LC_COLLATE=C              
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
## 
## time zone: Etc/UTC
## tzcode source: system (glibc)
## 
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## 
## other attached packages:
##  [1] crisprViz_1.12.0       crisprDesign_1.12.0    crisprScore_1.14.0    
##  [4] crisprScoreData_1.14.0 ExperimentHub_3.0.0    AnnotationHub_4.0.0   
##  [7] BiocFileCache_3.0.0    dbplyr_2.5.1           BiocGenerics_0.56.0   
## [10] generics_0.1.4         crisprBowtie_1.14.0    crisprBase_1.14.0     
## [13] crisprVerse_1.12.0     BiocStyle_2.38.0      
## 
## loaded via a namespace (and not attached):
##   [1] RColorBrewer_1.1-3          rstudioapi_0.17.1          
##   [3] sys_3.4.3                   jsonlite_2.0.0             
##   [5] magrittr_2.0.4              GenomicFeatures_1.62.0     
##   [7] farver_2.1.2                rmarkdown_2.30             
##   [9] BiocIO_1.20.0               vctrs_0.6.5                
##  [11] memoise_2.0.1               Rsamtools_2.26.0           
##  [13] RCurl_1.98-1.17             base64enc_0.1-3            
##  [15] htmltools_0.5.8.1           S4Arrays_1.10.0            
##  [17] progress_1.2.3              curl_7.0.0                 
##  [19] SparseArray_1.10.1          Formula_1.2-5              
##  [21] sass_0.4.10                 bslib_0.9.0                
##  [23] htmlwidgets_1.6.4           basilisk_1.22.0            
##  [25] Gviz_1.54.0                 httr2_1.2.1                
##  [27] cachem_1.1.0                buildtools_1.0.0           
##  [29] GenomicAlignments_1.46.0    lifecycle_1.0.4            
##  [31] pkgconfig_2.0.3             Matrix_1.7-4               
##  [33] R6_2.6.1                    fastmap_1.2.0              
##  [35] MatrixGenerics_1.22.0       digest_0.6.38              
##  [37] colorspace_2.1-2            AnnotationDbi_1.72.0       
##  [39] S4Vectors_0.48.0            Hmisc_5.2-4                
##  [41] GenomicRanges_1.62.0        RSQLite_2.4.4              
##  [43] filelock_1.0.3              randomForest_4.7-1.2       
##  [45] httr_1.4.7                  abind_1.4-8                
##  [47] compiler_4.5.2              Rbowtie_1.50.0             
##  [49] bit64_4.6.0-1               backports_1.5.0            
##  [51] htmlTable_2.4.3             S7_0.2.1                   
##  [53] BiocParallel_1.44.0         DBI_1.2.3                  
##  [55] biomaRt_2.66.0              rappdirs_0.3.3             
##  [57] DelayedArray_0.36.0         rjson_0.2.23               
##  [59] tools_4.5.2                 foreign_0.8-90             
##  [61] nnet_7.3-20                 glue_1.8.0                 
##  [63] restfulr_0.0.16             grid_4.5.2                 
##  [65] checkmate_2.3.3             cluster_2.1.8.1            
##  [67] gtable_0.3.6                BSgenome_1.78.0            
##  [69] tzdb_0.5.0                  ensembldb_2.34.0           
##  [71] data.table_1.17.8           hms_1.1.4                  
##  [73] XVector_0.50.0              BiocVersion_3.23.1         
##  [75] pillar_1.11.1               stringr_1.6.0              
##  [77] dplyr_1.1.4                 lattice_0.22-7             
##  [79] deldir_2.0-4                rtracklayer_1.70.0         
##  [81] bit_4.6.0                   biovizBase_1.58.0          
##  [83] tidyselect_1.2.1            maketools_1.3.2            
##  [85] Biostrings_2.78.0           knitr_1.50                 
##  [87] gridExtra_2.3               ProtGenerics_1.42.0        
##  [89] IRanges_2.44.0              Seqinfo_1.0.0              
##  [91] SummarizedExperiment_1.40.0 stats4_4.5.2               
##  [93] xfun_0.54                   Biobase_2.70.0             
##  [95] matrixStats_1.5.0           stringi_1.8.7              
##  [97] UCSC.utils_1.6.0            lazyeval_0.2.2             
##  [99] yaml_2.3.10                 evaluate_1.0.5             
## [101] codetools_0.2-20            cigarillo_1.0.0            
## [103] interp_1.1-6                tibble_3.3.0               
## [105] BiocManager_1.30.27         cli_3.6.5                  
## [107] rpart_4.1.24                reticulate_1.44.1          
## [109] jquerylib_0.1.4             dichromat_2.0-0.1          
## [111] Rcpp_1.1.0                  GenomeInfoDb_1.46.0        
## [113] dir.expiry_1.18.0           png_0.1-8                  
## [115] XML_3.99-0.20               parallel_4.5.2             
## [117] ggplot2_4.0.1               readr_2.1.6                
## [119] blob_1.2.4                  prettyunits_1.2.0          
## [121] jpeg_0.1-11                 latticeExtra_0.6-31        
## [123] AnnotationFilter_1.34.0     bitops_1.0-9               
## [125] txdbmaker_1.6.0             VariantAnnotation_1.56.0   
## [127] scales_1.4.0                crayon_1.5.3               
## [129] rlang_1.1.6                 KEGGREST_1.50.0