This page was generated on 2020-04-15 12:49:45 -0400 (Wed, 15 Apr 2020).
R version 3.6.3 (2020-02-29) -- "Holding the Windsock"
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> library(testthat)
> library(Maaslin2)
>
> test_check("Maaslin2")
[1] "Creating output folder"
2020-04-15 03:32:33 INFO::Writing function arguments to log file
2020-04-15 03:32:33 INFO::Verifying options selected are valid
2020-04-15 03:32:33 INFO::Determining format of input files
2020-04-15 03:32:33 INFO::Input format is data samples as rows and metadata samples as rows
2020-04-15 03:32:33 INFO::Formula for random effects: expr ~ (1 | site) + (1 | subject)
2020-04-15 03:32:33 INFO::Formula for fixed effects: expr ~ diagnosis + dysbiosisnonIBD + dysbiosisUC + dysbiosisCD + antibiotics + age
2020-04-15 03:32:33 INFO::Running selected normalization method: TSS
2020-04-15 03:32:35 INFO::Filter data based on min abundance and min prevalence
2020-04-15 03:32:35 INFO::Total samples in data: 1595
2020-04-15 03:32:35 INFO::Min samples required with min abundance for a feature not to be filtered: 159.500000
2020-04-15 03:32:35 INFO::Total filtered features: 0
2020-04-15 03:32:35 INFO::Filtered feature names:
2020-04-15 03:32:35 INFO::Bypass z-score application to metadata
2020-04-15 03:32:35 INFO::Running selected transform method: AST
2020-04-15 03:32:35 INFO::Running selected analysis method: LM
2020-04-15 03:32:35 INFO::Fitting model to feature number 1, Bifidobacterium.adolescentis
2020-04-15 03:32:36 INFO::Fitting model to feature number 2, Bifidobacterium.bifidum
2020-04-15 03:32:36 INFO::Fitting model to feature number 3, Bifidobacterium.longum
2020-04-15 03:32:37 INFO::Fitting model to feature number 4, Bifidobacterium.pseudocatenulatum
2020-04-15 03:32:37 INFO::Fitting model to feature number 5, Collinsella.aerofaciens
2020-04-15 03:32:37 INFO::Fitting model to feature number 6, Bacteroides.caccae
2020-04-15 03:32:38 INFO::Fitting model to feature number 7, Bacteroides.cellulosilyticus
2020-04-15 03:32:38 INFO::Fitting model to feature number 8, Bacteroides.dorei
2020-04-15 03:32:38 INFO::Fitting model to feature number 9, Bacteroides.eggerthii
2020-04-15 03:32:38 INFO::Fitting model to feature number 10, Bacteroides.faecis
2020-04-15 03:32:39 INFO::Fitting model to feature number 11, Bacteroides.finegoldii
2020-04-15 03:32:39 INFO::Fitting model to feature number 12, Bacteroides.fragilis
2020-04-15 03:32:39 INFO::Fitting model to feature number 13, Bacteroides.intestinalis
2020-04-15 03:32:40 INFO::Fitting model to feature number 14, Bacteroides.massiliensis
2020-04-15 03:32:40 INFO::Fitting model to feature number 15, Bacteroides.ovatus
2020-04-15 03:32:40 INFO::Fitting model to feature number 16, Bacteroides.salyersiae
2020-04-15 03:32:41 INFO::Fitting model to feature number 17, Bacteroides.stercoris
2020-04-15 03:32:41 INFO::Fitting model to feature number 18, Bacteroides.thetaiotaomicron
2020-04-15 03:32:41 INFO::Fitting model to feature number 19, Bacteroides.uniformis
2020-04-15 03:32:42 INFO::Fitting model to feature number 20, Bacteroides.vulgatus
2020-04-15 03:32:42 INFO::Fitting model to feature number 21, Bacteroides.xylanisolvens
2020-04-15 03:32:42 INFO::Fitting model to feature number 22, Bacteroidales.bacterium.ph8
2020-04-15 03:32:42 INFO::Fitting model to feature number 23, Barnesiella.intestinihominis
2020-04-15 03:32:43 INFO::Fitting model to feature number 24, Coprobacter.fastidiosus
2020-04-15 03:32:43 INFO::Fitting model to feature number 25, Odoribacter.splanchnicus
2020-04-15 03:32:43 INFO::Fitting model to feature number 26, Parabacteroides.distasonis
2020-04-15 03:32:44 INFO::Fitting model to feature number 27, Parabacteroides.goldsteinii
2020-04-15 03:32:44 INFO::Fitting model to feature number 28, Parabacteroides.merdae
2020-04-15 03:32:44 INFO::Fitting model to feature number 29, Parabacteroides.unclassified
2020-04-15 03:32:44 INFO::Fitting model to feature number 30, Paraprevotella.clara
2020-04-15 03:32:45 INFO::Fitting model to feature number 31, Paraprevotella.unclassified
2020-04-15 03:32:45 INFO::Fitting model to feature number 32, Prevotella.copri
2020-04-15 03:32:45 INFO::Fitting model to feature number 33, Alistipes.finegoldii
2020-04-15 03:32:46 INFO::Fitting model to feature number 34, Alistipes.onderdonkii
2020-04-15 03:32:46 INFO::Fitting model to feature number 35, Alistipes.putredinis
2020-04-15 03:32:46 INFO::Fitting model to feature number 36, Alistipes.shahii
2020-04-15 03:32:46 INFO::Fitting model to feature number 37, Alistipes.unclassified
2020-04-15 03:32:47 INFO::Fitting model to feature number 38, Streptococcus.salivarius
2020-04-15 03:32:47 INFO::Fitting model to feature number 39, Clostridium.bolteae
2020-04-15 03:32:47 INFO::Fitting model to feature number 40, Clostridium.citroniae
2020-04-15 03:32:47 INFO::Fitting model to feature number 41, Clostridium.clostridioforme
2020-04-15 03:32:48 INFO::Fitting model to feature number 42, Clostridium.hathewayi
2020-04-15 03:32:48 INFO::Fitting model to feature number 43, Clostridium.leptum
2020-04-15 03:32:48 INFO::Fitting model to feature number 44, Clostridium.nexile
2020-04-15 03:32:48 INFO::Fitting model to feature number 45, Clostridium.symbiosum
2020-04-15 03:32:49 INFO::Fitting model to feature number 46, Flavonifractor.plautii
2020-04-15 03:32:49 INFO::Fitting model to feature number 47, Eubacterium.eligens
2020-04-15 03:32:49 INFO::Fitting model to feature number 48, Eubacterium.hallii
2020-04-15 03:32:49 INFO::Fitting model to feature number 49, Eubacterium.rectale
2020-04-15 03:32:50 INFO::Fitting model to feature number 50, Eubacterium.siraeum
2020-04-15 03:32:50 INFO::Fitting model to feature number 51, Eubacterium.sp.3.1.31
2020-04-15 03:32:50 INFO::Fitting model to feature number 52, Eubacterium.ventriosum
2020-04-15 03:32:51 INFO::Fitting model to feature number 53, Ruminococcus.gnavus
2020-04-15 03:32:51 INFO::Fitting model to feature number 54, Ruminococcus.obeum
2020-04-15 03:32:51 INFO::Fitting model to feature number 55, Ruminococcus.torques
2020-04-15 03:32:51 INFO::Fitting model to feature number 56, Coprococcus.comes
2020-04-15 03:32:52 INFO::Fitting model to feature number 57, Dorea.longicatena
2020-04-15 03:32:52 INFO::Fitting model to feature number 58, Lachnospiraceae.bacterium.1.1.57FAA
2020-04-15 03:32:52 INFO::Fitting model to feature number 59, Lachnospiraceae.bacterium.3.1.46FAA
2020-04-15 03:32:53 INFO::Fitting model to feature number 60, Roseburia.hominis
2020-04-15 03:32:53 INFO::Fitting model to feature number 61, Roseburia.intestinalis
2020-04-15 03:32:53 INFO::Fitting model to feature number 62, Roseburia.inulinivorans
2020-04-15 03:32:53 INFO::Fitting model to feature number 63, Roseburia.unclassified
2020-04-15 03:32:54 INFO::Fitting model to feature number 64, Oscillibacter.unclassified
2020-04-15 03:32:54 INFO::Fitting model to feature number 65, Peptostreptococcaceae.noname.unclassified
2020-04-15 03:32:54 INFO::Fitting model to feature number 66, Faecalibacterium.prausnitzii
2020-04-15 03:32:54 INFO::Fitting model to feature number 67, Ruminococcus.bromii
2020-04-15 03:32:55 INFO::Fitting model to feature number 68, Ruminococcus.callidus
2020-04-15 03:32:55 INFO::Fitting model to feature number 69, Ruminococcus.lactaris
2020-04-15 03:32:55 INFO::Fitting model to feature number 70, Subdoligranulum.unclassified
2020-04-15 03:32:55 INFO::Fitting model to feature number 71, Coprobacillus.unclassified
2020-04-15 03:32:56 INFO::Fitting model to feature number 72, Acidaminococcus.unclassified
2020-04-15 03:32:56 INFO::Fitting model to feature number 73, Dialister.invisus
2020-04-15 03:32:56 INFO::Fitting model to feature number 74, Veillonella.atypica
2020-04-15 03:32:57 INFO::Fitting model to feature number 75, Veillonella.dispar
2020-04-15 03:32:57 INFO::Fitting model to feature number 76, Veillonella.parvula
2020-04-15 03:32:57 INFO::Fitting model to feature number 77, Veillonella.unclassified
2020-04-15 03:32:57 INFO::Fitting model to feature number 78, Burkholderiales.bacterium.1.1.47
2020-04-15 03:32:58 INFO::Fitting model to feature number 79, Parasutterella.excrementihominis
2020-04-15 03:32:58 INFO::Fitting model to feature number 80, Sutterella.wadsworthensis
2020-04-15 03:32:58 INFO::Fitting model to feature number 81, Bilophila.unclassified
2020-04-15 03:32:59 INFO::Fitting model to feature number 82, Escherichia.coli
2020-04-15 03:32:59 INFO::Fitting model to feature number 83, Escherichia.unclassified
2020-04-15 03:32:59 INFO::Fitting model to feature number 84, Klebsiella.pneumoniae
2020-04-15 03:33:00 INFO::Fitting model to feature number 85, Haemophilus.parainfluenzae
2020-04-15 03:33:00 INFO::Fitting model to feature number 86, Akkermansia.muciniphila
2020-04-15 03:33:00 INFO::Fitting model to feature number 87, C2likevirus.unclassified
2020-04-15 03:33:00 INFO::Counting total values for each feature
2020-04-15 03:33:01 INFO::Writing residuals to file output/residuals.rds
2020-04-15 03:33:01 INFO::Writing all results to file (ordered by increasing q-values): output/all_results.tsv
2020-04-15 03:33:01 INFO::Writing the significant results (those which are less than or equal to the threshold of 0.250000 ) to file (ordered by increasing q-values): output/significant_results.tsv
── 1. Failure: (unknown) (@test_Maaslin2.R#17) ────────────────────────────────
`x` not equal to `expected`.
5/208 mismatches
x[133]: "dysbiosisUC"
y[133]: "diagnosis"
x[134]: "dysbiosisnonIBD"
y[134]: "dysbiosisUC"
x[135]: "diagnosis"
y[135]: "dysbiosisnonIBD"
x[185]: "antibiotics"
y[185]: "diagnosis"
x[187]: "diagnosis"
y[187]: "antibiotics"
── 2. Failure: (unknown) (@test_Maaslin2.R#18) ────────────────────────────────
`x` not equal to `expected`.
10/208 mismatches
x[133]: "Clostridium.nexile"
y[133]: "Ruminococcus.callidus"
x[134]: "Lachnospiraceae.bacterium.3.1.46FAA"
y[134]: "Clostridium.nexile"
x[135]: "Ruminococcus.callidus"
y[135]: "Lachnospiraceae.bacterium.3.1.46FAA"
x[185]: "Veillonella.parvula"
y[185]: "Ruminococcus.gnavus"
x[186]: "Ruminococcus.gnavus"
y[186]: "Ruminococcus.obeum"
── 3. Failure: (unknown) (@test_Maaslin2.R#20) ────────────────────────────────
`x` not equal to `expected`.
10/208 mismatches (average diff: 427)
[133] 273 - 323 == -50
[134] 992 - 273 == 719
[135] 323 - 992 == -669
[185] 853 - 970 == -117
[186] 970 - 1346 == -376
[187] 1346 - 1514 == -168
[188] 1514 - 415 == 1099
[189] 415 - 853 == -438
[203] 730 - 415 == 315
...
[1] "Creating output folder"
2020-04-15 03:33:01 INFO::Writing function arguments to log file
2020-04-15 03:33:01 INFO::Verifying options selected are valid
2020-04-15 03:33:01 INFO::Determining format of input files
2020-04-15 03:33:01 INFO::Input format is data samples as rows and metadata samples as rows
2020-04-15 03:33:01 INFO::Formula for random effects: expr ~ (1 | site) + (1 | subject)
2020-04-15 03:33:01 INFO::Formula for fixed effects: expr ~ diagnosis + dysbiosis + antibiotics + age
2020-04-15 03:33:01 INFO::Running selected normalization method: NONE
2020-04-15 03:33:01 INFO::Filter data based on min abundance and min prevalence
2020-04-15 03:33:01 INFO::Total samples in data: 1595
2020-04-15 03:33:01 INFO::Min samples required with min abundance for a feature not to be filtered: 159.500000
2020-04-15 03:33:01 INFO::Total filtered features: 0
2020-04-15 03:33:01 INFO::Filtered feature names:
2020-04-15 03:33:01 INFO::Bypass z-score application to metadata
2020-04-15 03:33:01 INFO::Running selected transform method: AST
2020-04-15 03:33:01 INFO::Running selected analysis method: LM
2020-04-15 03:33:01 INFO::Fitting model to feature number 1, Bifidobacterium.adolescentis
2020-04-15 03:33:01 INFO::Fitting model to feature number 2, Bifidobacterium.bifidum
2020-04-15 03:33:01 INFO::Fitting model to feature number 3, Bifidobacterium.longum
2020-04-15 03:33:01 INFO::Fitting model to feature number 4, Bifidobacterium.pseudocatenulatum
2020-04-15 03:33:02 INFO::Fitting model to feature number 5, Collinsella.aerofaciens
2020-04-15 03:33:02 INFO::Fitting model to feature number 6, Bacteroides.caccae
2020-04-15 03:33:02 INFO::Fitting model to feature number 7, Bacteroides.cellulosilyticus
2020-04-15 03:33:03 INFO::Fitting model to feature number 8, Bacteroides.dorei
2020-04-15 03:33:03 INFO::Fitting model to feature number 9, Bacteroides.eggerthii
2020-04-15 03:33:03 INFO::Fitting model to feature number 10, Bacteroides.faecis
2020-04-15 03:33:03 INFO::Fitting model to feature number 11, Bacteroides.finegoldii
2020-04-15 03:33:04 INFO::Fitting model to feature number 12, Bacteroides.fragilis
2020-04-15 03:33:04 INFO::Fitting model to feature number 13, Bacteroides.intestinalis
2020-04-15 03:33:04 INFO::Fitting model to feature number 14, Bacteroides.massiliensis
2020-04-15 03:33:04 INFO::Fitting model to feature number 15, Bacteroides.ovatus
2020-04-15 03:33:05 INFO::Fitting model to feature number 16, Bacteroides.salyersiae
2020-04-15 03:33:05 INFO::Fitting model to feature number 17, Bacteroides.stercoris
2020-04-15 03:33:05 INFO::Fitting model to feature number 18, Bacteroides.thetaiotaomicron
2020-04-15 03:33:05 INFO::Fitting model to feature number 19, Bacteroides.uniformis
2020-04-15 03:33:06 INFO::Fitting model to feature number 20, Bacteroides.vulgatus
2020-04-15 03:33:06 INFO::Fitting model to feature number 21, Bacteroides.xylanisolvens
2020-04-15 03:33:06 INFO::Fitting model to feature number 22, Bacteroidales.bacterium.ph8
2020-04-15 03:33:07 INFO::Fitting model to feature number 23, Barnesiella.intestinihominis
2020-04-15 03:33:07 INFO::Fitting model to feature number 24, Coprobacter.fastidiosus
2020-04-15 03:33:07 INFO::Fitting model to feature number 25, Odoribacter.splanchnicus
2020-04-15 03:33:07 INFO::Fitting model to feature number 26, Parabacteroides.distasonis
2020-04-15 03:33:08 INFO::Fitting model to feature number 27, Parabacteroides.goldsteinii
2020-04-15 03:33:08 INFO::Fitting model to feature number 28, Parabacteroides.merdae
2020-04-15 03:33:08 INFO::Fitting model to feature number 29, Parabacteroides.unclassified
2020-04-15 03:33:09 INFO::Fitting model to feature number 30, Paraprevotella.clara
2020-04-15 03:33:09 INFO::Fitting model to feature number 31, Paraprevotella.unclassified
2020-04-15 03:33:09 INFO::Fitting model to feature number 32, Prevotella.copri
2020-04-15 03:33:09 INFO::Fitting model to feature number 33, Alistipes.finegoldii
2020-04-15 03:33:10 INFO::Fitting model to feature number 34, Alistipes.onderdonkii
2020-04-15 03:33:10 INFO::Fitting model to feature number 35, Alistipes.putredinis
2020-04-15 03:33:10 INFO::Fitting model to feature number 36, Alistipes.shahii
2020-04-15 03:33:10 INFO::Fitting model to feature number 37, Alistipes.unclassified
2020-04-15 03:33:11 INFO::Fitting model to feature number 38, Streptococcus.salivarius
2020-04-15 03:33:11 INFO::Fitting model to feature number 39, Clostridium.bolteae
2020-04-15 03:33:11 INFO::Fitting model to feature number 40, Clostridium.citroniae
2020-04-15 03:33:12 INFO::Fitting model to feature number 41, Clostridium.clostridioforme
2020-04-15 03:33:12 INFO::Fitting model to feature number 42, Clostridium.hathewayi
2020-04-15 03:33:12 INFO::Fitting model to feature number 43, Clostridium.leptum
2020-04-15 03:33:13 INFO::Fitting model to feature number 44, Clostridium.nexile
2020-04-15 03:33:13 INFO::Fitting model to feature number 45, Clostridium.symbiosum
2020-04-15 03:33:13 INFO::Fitting model to feature number 46, Flavonifractor.plautii
2020-04-15 03:33:13 INFO::Fitting model to feature number 47, Eubacterium.eligens
2020-04-15 03:33:14 INFO::Fitting model to feature number 48, Eubacterium.hallii
2020-04-15 03:33:14 INFO::Fitting model to feature number 49, Eubacterium.rectale
2020-04-15 03:33:14 INFO::Fitting model to feature number 50, Eubacterium.siraeum
2020-04-15 03:33:15 INFO::Fitting model to feature number 51, Eubacterium.sp.3.1.31
2020-04-15 03:33:15 INFO::Fitting model to feature number 52, Eubacterium.ventriosum
2020-04-15 03:33:15 INFO::Fitting model to feature number 53, Ruminococcus.gnavus
2020-04-15 03:33:15 INFO::Fitting model to feature number 54, Ruminococcus.obeum
2020-04-15 03:33:16 INFO::Fitting model to feature number 55, Ruminococcus.torques
2020-04-15 03:33:16 INFO::Fitting model to feature number 56, Coprococcus.comes
2020-04-15 03:33:16 INFO::Fitting model to feature number 57, Dorea.longicatena
2020-04-15 03:33:17 INFO::Fitting model to feature number 58, Lachnospiraceae.bacterium.1.1.57FAA
2020-04-15 03:33:17 INFO::Fitting model to feature number 59, Lachnospiraceae.bacterium.3.1.46FAA
2020-04-15 03:33:17 INFO::Fitting model to feature number 60, Roseburia.hominis
2020-04-15 03:33:18 INFO::Fitting model to feature number 61, Roseburia.intestinalis
2020-04-15 03:33:18 INFO::Fitting model to feature number 62, Roseburia.inulinivorans
2020-04-15 03:33:18 INFO::Fitting model to feature number 63, Roseburia.unclassified
2020-04-15 03:33:19 INFO::Fitting model to feature number 64, Oscillibacter.unclassified
2020-04-15 03:33:19 INFO::Fitting model to feature number 65, Peptostreptococcaceae.noname.unclassified
2020-04-15 03:33:19 INFO::Fitting model to feature number 66, Faecalibacterium.prausnitzii
2020-04-15 03:33:19 INFO::Fitting model to feature number 67, Ruminococcus.bromii
2020-04-15 03:33:19 INFO::Fitting model to feature number 68, Ruminococcus.callidus
2020-04-15 03:33:20 INFO::Fitting model to feature number 69, Ruminococcus.lactaris
2020-04-15 03:33:20 INFO::Fitting model to feature number 70, Subdoligranulum.unclassified
2020-04-15 03:33:20 INFO::Fitting model to feature number 71, Coprobacillus.unclassified
2020-04-15 03:33:20 INFO::Fitting model to feature number 72, Acidaminococcus.unclassified
2020-04-15 03:33:21 INFO::Fitting model to feature number 73, Dialister.invisus
2020-04-15 03:33:21 INFO::Fitting model to feature number 74, Veillonella.atypica
2020-04-15 03:33:21 INFO::Fitting model to feature number 75, Veillonella.dispar
2020-04-15 03:33:21 INFO::Fitting model to feature number 76, Veillonella.parvula
2020-04-15 03:33:22 INFO::Fitting model to feature number 77, Veillonella.unclassified
2020-04-15 03:33:22 INFO::Fitting model to feature number 78, Burkholderiales.bacterium.1.1.47
2020-04-15 03:33:22 INFO::Fitting model to feature number 79, Parasutterella.excrementihominis
2020-04-15 03:33:22 INFO::Fitting model to feature number 80, Sutterella.wadsworthensis
2020-04-15 03:33:22 INFO::Fitting model to feature number 81, Bilophila.unclassified
2020-04-15 03:33:23 INFO::Fitting model to feature number 82, Escherichia.coli
2020-04-15 03:33:23 INFO::Fitting model to feature number 83, Escherichia.unclassified
2020-04-15 03:33:23 INFO::Fitting model to feature number 84, Klebsiella.pneumoniae
2020-04-15 03:33:24 INFO::Fitting model to feature number 85, Haemophilus.parainfluenzae
2020-04-15 03:33:24 INFO::Fitting model to feature number 86, Akkermansia.muciniphila
2020-04-15 03:33:24 INFO::Fitting model to feature number 87, C2likevirus.unclassified
2020-04-15 03:33:24 INFO::Counting total values for each feature
2020-04-15 03:33:24 INFO::Writing residuals to file output2/residuals.rds
2020-04-15 03:33:25 INFO::Writing all results to file (ordered by increasing q-values): output2/all_results.tsv
2020-04-15 03:33:25 INFO::Writing the significant results (those which are less than or equal to the threshold of 0.250000 ) to file (ordered by increasing q-values): output2/significant_results.tsv
══ testthat results ═══════════════════════════════════════════════════════════
[ OK: 9 | SKIPPED: 0 | WARNINGS: 6 | FAILED: 3 ]
1. Failure: (unknown) (@test_Maaslin2.R#17)
2. Failure: (unknown) (@test_Maaslin2.R#18)
3. Failure: (unknown) (@test_Maaslin2.R#20)
Error: testthat unit tests failed
Execution halted