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This page was generated on 2026-04-27 11:33 -0400 (Mon, 27 Apr 2026).

HostnameOSArch (*)R versionInstalled pkgs
nebbiolo2Linux (Ubuntu 24.04.4 LTS)x86_644.6.0 RC (2026-04-17 r89917) -- "Because it was There" 4822
Click on any hostname to see more info about the system (e.g. compilers)      (*) as reported by 'uname -p', except on Windows and Mac OS X

Package 1174/2365HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
maaslin3 1.3.1  (landing page)
William Nickols
Snapshot Date: 2026-04-26 13:45 -0400 (Sun, 26 Apr 2026)
git_url: https://git.bioconductor.org/packages/maaslin3
git_branch: devel
git_last_commit: 1423bd7
git_last_commit_date: 2026-04-24 11:47:11 -0400 (Fri, 24 Apr 2026)
nebbiolo2Linux (Ubuntu 24.04.4 LTS) / x86_64  OK    OK    OK  UNNEEDED, same version is already published
See other builds for maaslin3 in R Universe.


CHECK results for maaslin3 on nebbiolo2

To the developers/maintainers of the maaslin3 package:
- Allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/maaslin3.git to reflect on this report. See Troubleshooting Build Report for more information.
- Use the following Renviron settings to reproduce errors and warnings.
- If 'R CMD check' started to fail recently on the Linux builder(s) over a missing dependency, add the missing dependency to 'Suggests:' in your DESCRIPTION file. See Renviron.bioc for more information.

raw results


Summary

Package: maaslin3
Version: 1.3.1
Command: /home/biocbuild/bbs-3.24-bioc/R/bin/R CMD check --install=check:maaslin3.install-out.txt --library=/home/biocbuild/bbs-3.24-bioc/R/site-library --timings maaslin3_1.3.1.tar.gz
StartedAt: 2026-04-27 00:59:54 -0400 (Mon, 27 Apr 2026)
EndedAt: 2026-04-27 01:15:46 -0400 (Mon, 27 Apr 2026)
EllapsedTime: 951.1 seconds
RetCode: 0
Status:   OK  
CheckDir: maaslin3.Rcheck
Warnings: 0

Command output

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.24-bioc/R/bin/R CMD check --install=check:maaslin3.install-out.txt --library=/home/biocbuild/bbs-3.24-bioc/R/site-library --timings maaslin3_1.3.1.tar.gz
###
##############################################################################
##############################################################################


* using log directory ‘/home/biocbuild/bbs-3.24-bioc/meat/maaslin3.Rcheck’
* using R version 4.6.0 RC (2026-04-17 r89917)
* using platform: x86_64-pc-linux-gnu
* R was compiled by
    gcc (Ubuntu 13.3.0-6ubuntu2~24.04.1) 13.3.0
    GNU Fortran (Ubuntu 13.3.0-6ubuntu2~24.04.1) 13.3.0
* running under: Ubuntu 24.04.4 LTS
* using session charset: UTF-8
* current time: 2026-04-27 04:59:55 UTC
* checking for file ‘maaslin3/DESCRIPTION’ ... OK
* this is package ‘maaslin3’ version ‘1.3.1’
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘maaslin3’ can be installed ... OK
* checking installed package size ... OK
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... NOTE
License stub is invalid DCF.
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking code files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking loading without being on the library search path ... OK
* checking dependencies in R code ... NOTE
Unexported objects imported by ':::' calls:
  ‘lmerTest:::summary.lmerModLmerTest’ ‘survival:::summary.coxph’
  See the note in ?`:::` about the use of this operator.
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... NOTE
Found the following Rd file(s) with Rd \link{} targets missing package
anchors:
  maaslin3.Rd: mirai
  maaslin_fit.Rd: mirai
  maaslin_log_arguments.Rd: mirai
Please provide package anchors for all Rd \link{} targets not in the
package itself and the base packages.
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking files in ‘vignettes’ ... OK
* checking examples ... OK
Examples with CPU (user + system) or elapsed time > 5s
                                     user system elapsed
maaslin_plot_results               39.625  0.521  40.970
maaslin_plot_results_from_output   35.047  0.352  35.388
maaslin3                           17.485  0.676  18.148
maaslin_contrast_test              17.213  0.348  17.549
maaslin_write_results_lefse_format 14.554  0.177  14.720
maaslin_fit                        13.697  0.216  14.686
maaslin_write_results              13.438  0.202  13.630
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘testthat.R’
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes ... OK
* checking re-building of vignette outputs ... OK
* checking PDF version of manual ... OK
* DONE

Status: 3 NOTEs
See
  ‘/home/biocbuild/bbs-3.24-bioc/meat/maaslin3.Rcheck/00check.log’
for details.


Installation output

maaslin3.Rcheck/00install.out

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.24-bioc/R/bin/R CMD INSTALL maaslin3
###
##############################################################################
##############################################################################


* installing to library ‘/home/biocbuild/bbs-3.24-bioc/R/site-library’
* installing *source* package ‘maaslin3’ ...
** this is package ‘maaslin3’ version ‘1.3.1’
** using staged installation
** R
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (maaslin3)

Tests output

maaslin3.Rcheck/tests/testthat.Rout


R version 4.6.0 RC (2026-04-17 r89917) -- "Because it was There"
Copyright (C) 2026 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(testthat)
> library(maaslin3)
> 
> test_check("maaslin3")
Warning: stack imbalance in '::', 52 then 54
Warning: stack imbalance in '<-', 50 then 52
2026-04-27 01:05:43.06 ERROR::Please select a normalization from the list of available options : TSS, CLR, NONE
2026-04-27 01:05:43.08 ERROR::Please select a transform from the list of available options : LOG, PLOG, NONE
2026-04-27 01:05:43.09 ERROR::Please select a correction method from the list of available options : BH, holm, hochberg, hommel, bonferroni, BY
2026-04-27 01:05:43.14 ERROR::No fixed, group, or
                        ordered effects included in formula.
2026-04-27 01:05:43.15 ERROR::Effect name not found in metadata: d
2026-04-27 01:05:43.15 ERROR::No user formula provided
2026-04-27 01:05:43.18 ERROR::Effect name not found in metadata so not applied to formula as random effect: d
2026-04-27 01:05:43.19 ERROR::No fixed/group/ordered/
                        feature-specific effects provided.
2026-04-27 01:05:45.75 INFO::Writing function arguments to log file
2026-04-27 01:05:45.77 INFO::Verifying options selected are valid
2026-04-27 01:05:50.01 INFO::Writing function arguments to log file
2026-04-27 01:05:50.03 INFO::Verifying options selected are valid
2026-04-27 01:05:50.03 INFO::Determining format of input files
2026-04-27 01:05:50.03 INFO::Input format is data samples as rows and metadata samples as rows
2026-04-27 01:05:50.04 INFO::Running selected normalization method: TSS
2026-04-27 01:05:50.04 INFO::Creating output feature tables folder
2026-04-27 01:05:50.04 INFO::Writing normalized data to file /tmp/RtmpdBrYAP/file3c6585727d523/features/data_norm.tsv
2026-04-27 01:05:50.04 INFO::Filter data based on min abundance, min prevalence, and max prevalence
2026-04-27 01:05:50.04 INFO::Total samples in data: 16
2026-04-27 01:05:50.05 INFO::Min samples required with min abundance for a feature not to be filtered: 0.000000
2026-04-27 01:05:50.05 INFO::Max samples allowed with min abundance for a feature not to be filtered: 16.160000
2026-04-27 01:05:50.05 INFO::Total filtered features: 0
2026-04-27 01:05:50.05 INFO::Filtered feature names from abundance, min prevalence, and max prevalence filtering:
2026-04-27 01:05:50.05 INFO::Total features filtered by non-zero variance filtering: 0
2026-04-27 01:05:50.05 INFO::Filtered feature names from variance filtering:
2026-04-27 01:05:50.06 INFO::Writing filtered data to file /tmp/RtmpdBrYAP/file3c6585727d523/features/filtered_data.tsv
2026-04-27 01:05:50.06 INFO::Running selected transform method: LOG
2026-04-27 01:05:50.06 INFO::Writing normalized, filtered, transformed data to file /tmp/RtmpdBrYAP/file3c6585727d523/features/data_transformed.tsv
2026-04-27 01:05:50.06 INFO::Applying z-score to standardize continuous metadata
2026-04-27 01:05:50.06 INFO::Running the linear model component
2026-04-27 01:05:50.09 INFO::Fitting model to feature number 1, a
2026-04-27 01:05:50.09 INFO::Fitting model to feature number 2, b
2026-04-27 01:05:50.10 INFO::Fitting model to feature number 3, c
2026-04-27 01:05:50.10 INFO::Counting total values for each feature
2026-04-27 01:05:50.11 INFO::Running the logistic model component
2026-04-27 01:05:50.13 INFO::Fitting model to feature number 1, a
2026-04-27 01:05:50.14 INFO::Fitting model to feature number 2, b
2026-04-27 01:05:50.14 INFO::Fitting model to feature number 3, c
2026-04-27 01:05:50.15 INFO::Counting total values for each feature
2026-04-27 01:05:50.15 INFO::Re-running abundances for warn_prevalence
2026-04-27 01:05:50.16 INFO::Running selected normalization method: TSS
2026-04-27 01:05:50.16 INFO::Running selected transform method: LOG
2026-04-27 01:05:50.18 INFO::Fitting model to feature number 1, a
2026-04-27 01:05:50.19 INFO::Fitting model to feature number 2, b
2026-04-27 01:05:50.19 INFO::Fitting model to feature number 3, c
2026-04-27 01:05:50.20 INFO::Creating fits folder
2026-04-27 01:05:50.20 INFO::Writing residuals to file /tmp/RtmpdBrYAP/file3c6585727d523/fits/residuals_linear.rds
2026-04-27 01:05:50.21 INFO::Writing fitted values to file /tmp/RtmpdBrYAP/file3c6585727d523/fits/fitted_linear.rds
2026-04-27 01:05:50.21 INFO::Writing residuals to file /tmp/RtmpdBrYAP/file3c6585727d523/fits/residuals_logistic.rds
2026-04-27 01:05:50.21 INFO::Writing fitted values to file /tmp/RtmpdBrYAP/file3c6585727d523/fits/fitted_logistic.rds
2026-04-27 01:05:50.21 INFO::Writing all the results to file (ordered 
            by increasing individual q-values): /tmp/RtmpdBrYAP/file3c6585727d523/all_results.tsv
2026-04-27 01:05:50.22 INFO::Writing the significant results without errors (those which have joint q-values less than or equal to the threshold of 0.100000 ) to file (ordered by increasing individual q-values): /tmp/RtmpdBrYAP/file3c6585727d523/significant_results.tsv
2026-04-27 01:05:50.22 INFO::Creating output figures folder
2026-04-27 01:05:50.22 INFO::Writing summary plot of significant
                        results to file: /tmp/RtmpdBrYAP/file3c6585727d523/figures/summary_plot.pdf
2026-04-27 01:05:51.80 INFO::Writing association plots (one for each significant association) to output folder: /tmp/RtmpdBrYAP/file3c6585727d523/figures
2026-04-27 01:05:51.80 INFO::Plotting associations from most to least significant, grouped by metadata
2026-04-27 01:05:51.81 INFO::Creating boxplot for continuous data (logistic), var1 vs a
2026-04-27 01:05:52.34 INFO::Writing summary plot of
                        significant results to file: /tmp/RtmpdBrYAP/file3c6585727d523/figures/summary_plot.pdf
2026-04-27 01:05:54.02 INFO::Writing association plots (one for each significant association) to output folder: /tmp/RtmpdBrYAP/file3c6585727d523/figures
2026-04-27 01:05:54.02 INFO::Plotting associations from most to least significant, grouped by metadata
2026-04-27 01:05:54.03 INFO::Creating boxplot for continuous data (logistic), var1 vs a
2026-04-27 01:05:54.55 INFO::Running the linear model component
2026-04-27 01:05:54.56 INFO::Fitting model to feature number 1, a
2026-04-27 01:05:54.56 INFO::Fitting model to feature number 2, b
2026-04-27 01:05:54.57 INFO::Fitting model to feature number 3, c
2026-04-27 01:05:54.57 INFO::Counting total values for each feature
2026-04-27 01:05:54.58 INFO::Running the logistic model component
2026-04-27 01:05:54.59 INFO::Fitting model to feature number 1, a
2026-04-27 01:05:54.59 INFO::Fitting model to feature number 2, b
2026-04-27 01:05:54.60 INFO::Fitting model to feature number 3, c
2026-04-27 01:05:54.61 INFO::Counting total values for each feature
2026-04-27 01:05:54.61 INFO::Re-running abundances for warn_prevalence
2026-04-27 01:05:54.61 INFO::Running selected normalization method: TSS
2026-04-27 01:05:54.61 INFO::Running selected transform method: LOG
2026-04-27 01:05:54.62 INFO::Fitting model to feature number 1, a
2026-04-27 01:05:54.63 INFO::Fitting model to feature number 2, b
2026-04-27 01:05:54.63 INFO::Fitting model to feature number 3, c
2026-04-27 01:05:54.64 INFO::Creating output folder
2026-04-27 01:05:54.64 INFO::Creating output figures folder
2026-04-27 01:05:54.64 INFO::Writing summary plot of significant
                        results to file: /tmp/RtmpdBrYAP/file3c6585f713895/figures/summary_plot.pdf
2026-04-27 01:05:56.26 INFO::Writing association plots (one for each significant association) to output folder: /tmp/RtmpdBrYAP/file3c6585f713895/figures
2026-04-27 01:05:56.26 INFO::Plotting associations from most to least significant, grouped by metadata
2026-04-27 01:05:56.26 INFO::Creating boxplot for continuous data (logistic), var1 vs a
2026-04-27 01:05:56.79 INFO::Applying z-score to standardize continuous metadata
2026-04-27 01:05:56.79 INFO::Factor detected for categorial metadata 'c'. Using as-is.
2026-04-27 01:05:56.80 INFO::Bypass z-score application to metadata
2026-04-27 01:05:56.80 INFO::Bypass z-score application to metadata
2026-04-27 01:05:56.84 INFO::Determining format of input files
2026-04-27 01:05:56.84 INFO::Input format is data samples as rows and metadata samples as rows
2026-04-27 01:05:56.84 INFO::Input format is data samples as rows and feature_specific_covariate samples as rows
2026-04-27 01:05:56.85 INFO::Determining format of input files
2026-04-27 01:05:56.85 INFO::Input format is data samples as columns and metadata samples as rows
2026-04-27 01:05:56.86 INFO::Input format is feature_specific_covariate samples as columns
2026-04-27 01:05:56.86 INFO::Determining format of input files
2026-04-27 01:05:56.87 INFO::Input format is data samples as rows and metadata samples as rows
2026-04-27 01:05:56.87 INFO::Input format is feature_specific_covariate samples as columns
2026-04-27 01:05:56.87 INFO::The following samples were found to have metadata but no features (or feature specific covariates if applicable). They will be removed. sample1
2026-04-27 01:05:56.87 INFO::The following samples were found to have feature specific covariates but no features or no metadata. They will be removed. sample1
2026-04-27 01:05:56.88 INFO::Running selected transform method: LOG
2026-04-27 01:05:56.88 INFO::Creating output feature tables folder
2026-04-27 01:05:56.88 INFO::Writing normalized, filtered, transformed data to file /tmp/RtmpdBrYAP/file3c65854f958b1b/features/data_transformed.tsv
2026-04-27 01:05:56.89 INFO::Running selected transform method: LOG
2026-04-27 01:05:56.89 INFO::Writing normalized, filtered, transformed data to file /tmp/RtmpdBrYAP/file3c65854f958b1b/features/data_transformed.tsv
2026-04-27 01:05:56.89 INFO::Running selected transform method: PLOG
2026-04-27 01:05:56.90 INFO::Writing normalized, filtered, transformed data to file /tmp/RtmpdBrYAP/file3c65854f958b1b/features/data_transformed.tsv
2026-04-27 01:05:56.90 INFO::Running selected transform method: NONE
2026-04-27 01:05:56.90 INFO::Writing normalized, filtered, transformed data to file /tmp/RtmpdBrYAP/file3c65854f958b1b/features/data_transformed.tsv
2026-04-27 01:05:56.91 INFO::Running the linear model component
2026-04-27 01:05:56.92 INFO::Fitting model to feature number 1, a
2026-04-27 01:05:56.93 INFO::Fitting model to feature number 2, b
2026-04-27 01:05:56.93 INFO::Fitting model to feature number 3, c
2026-04-27 01:05:56.94 INFO::Counting total values for each feature
2026-04-27 01:05:56.94 INFO::Running the logistic model component
2026-04-27 01:05:56.95 INFO::Fitting model to feature number 1, a
2026-04-27 01:05:56.95 INFO::Fitting model to feature number 2, b
2026-04-27 01:05:56.96 INFO::Fitting model to feature number 3, c
2026-04-27 01:05:56.97 INFO::Counting total values for each feature
2026-04-27 01:05:56.97 INFO::Re-running abundances for warn_prevalence
2026-04-27 01:05:56.97 INFO::Running selected normalization method: TSS
2026-04-27 01:05:56.97 INFO::Running selected transform method: LOG
2026-04-27 01:05:56.98 INFO::Fitting model to feature number 1, a
2026-04-27 01:05:56.99 INFO::Fitting model to feature number 2, b
2026-04-27 01:05:56.99 INFO::Fitting model to feature number 3, c
2026-04-27 01:05:57.00 INFO::Creating output folder
2026-04-27 01:05:57.01 INFO::Creating fits folder
2026-04-27 01:05:57.01 INFO::Writing residuals to file /tmp/RtmpdBrYAP/file3c6585416ae29c/fits/residuals_linear.rds
2026-04-27 01:05:57.01 INFO::Writing fitted values to file /tmp/RtmpdBrYAP/file3c6585416ae29c/fits/fitted_linear.rds
2026-04-27 01:05:57.01 INFO::Writing residuals to file /tmp/RtmpdBrYAP/file3c6585416ae29c/fits/residuals_logistic.rds
2026-04-27 01:05:57.01 INFO::Writing fitted values to file /tmp/RtmpdBrYAP/file3c6585416ae29c/fits/fitted_logistic.rds
2026-04-27 01:05:57.01 INFO::Writing all the results to file (ordered 
            by increasing individual q-values): /tmp/RtmpdBrYAP/file3c6585416ae29c/all_results.tsv
2026-04-27 01:05:57.01 INFO::Writing the significant results without errors (those which have joint q-values less than or equal to the threshold of 0.100000 ) to file (ordered by increasing individual q-values): /tmp/RtmpdBrYAP/file3c6585416ae29c/significant_results.tsv
2026-04-27 01:05:57.65 INFO::Running the linear model component
2026-04-27 01:05:57.67 INFO::Fitting model to feature number 1, a
2026-04-27 01:05:57.67 INFO::Fitting model to feature number 2, b
2026-04-27 01:05:57.68 INFO::Fitting model to feature number 3, c
2026-04-27 01:05:57.68 INFO::Counting total values for each feature
2026-04-27 01:05:57.68 INFO::Running the logistic model component
2026-04-27 01:05:57.69 INFO::Fitting model to feature number 1, a
2026-04-27 01:05:57.70 INFO::Fitting model to feature number 2, b
2026-04-27 01:05:57.70 INFO::Fitting model to feature number 3, c
2026-04-27 01:05:57.71 INFO::Counting total values for each feature
2026-04-27 01:05:57.71 INFO::Re-running abundances for warn_prevalence
2026-04-27 01:05:57.72 INFO::Running selected normalization method: TSS
2026-04-27 01:05:57.72 INFO::Running selected transform method: LOG
2026-04-27 01:05:57.73 INFO::Fitting model to feature number 1, a
2026-04-27 01:05:57.73 INFO::Fitting model to feature number 2, b
2026-04-27 01:05:57.75 INFO::Fitting model to feature number 3, c
2026-04-27 01:05:58.71 INFO::Started 2 mirai daemons via `cores` argument
2026-04-27 01:05:58.71 INFO::Running the linear model component
2026-04-27 01:06:00.08 INFO::Counting total values for each feature
2026-04-27 01:06:00.08 INFO::Running the logistic model component
2026-04-27 01:06:00.17 INFO::Counting total values for each feature
2026-04-27 01:06:00.17 INFO::Re-running abundances for warn_prevalence
2026-04-27 01:06:00.17 INFO::Running selected normalization method: TSS
2026-04-27 01:06:00.17 INFO::Running selected transform method: LOG
2026-04-27 01:06:01.88 INFO::Running the linear model component
2026-04-27 01:06:03.28 INFO::Counting total values for each feature
2026-04-27 01:06:03.28 INFO::Running the logistic model component
2026-04-27 01:06:03.36 INFO::Counting total values for each feature
2026-04-27 01:06:03.37 INFO::Re-running abundances for warn_prevalence
2026-04-27 01:06:03.37 INFO::Running selected normalization method: TSS
2026-04-27 01:06:03.37 INFO::Running selected transform method: LOG
2026-04-27 01:06:05.58 INFO::Running the linear model component
2026-04-27 01:06:07.02 INFO::Counting total values for each feature
2026-04-27 01:06:07.03 INFO::Running the logistic model component
2026-04-27 01:06:07.11 INFO::Counting total values for each feature
2026-04-27 01:06:07.11 INFO::Re-running abundances for warn_prevalence
2026-04-27 01:06:07.12 INFO::Running selected normalization method: TSS
2026-04-27 01:06:07.12 INFO::Running selected transform method: LOG
2026-04-27 01:06:08.82 INFO::Writing function arguments to log file
2026-04-27 01:06:08.85 INFO::Verifying options selected are valid
2026-04-27 01:06:08.85 INFO::Determining format of input files
2026-04-27 01:06:08.85 INFO::Input format is data samples as rows and metadata samples as rows
2026-04-27 01:06:08.86 INFO::Running selected normalization method: TSS
2026-04-27 01:06:08.86 INFO::Creating output feature tables folder
2026-04-27 01:06:08.86 INFO::Writing normalized data to file /tmp/RtmpdBrYAP/maaslin3_parallel_test_3c65854af5e5bf/features/data_norm.tsv
2026-04-27 01:06:08.86 INFO::Filter data based on min abundance, min prevalence, and max prevalence
2026-04-27 01:06:08.87 INFO::Total samples in data: 30
2026-04-27 01:06:08.87 INFO::Min samples required with min abundance for a feature not to be filtered: 0.000000
2026-04-27 01:06:08.87 INFO::Max samples allowed with min abundance for a feature not to be filtered: 30.300000
2026-04-27 01:06:08.87 INFO::Total filtered features: 0
2026-04-27 01:06:08.87 INFO::Filtered feature names from abundance, min prevalence, and max prevalence filtering:
2026-04-27 01:06:08.87 INFO::Total features filtered by non-zero variance filtering: 0
2026-04-27 01:06:08.88 INFO::Filtered feature names from variance filtering:
2026-04-27 01:06:08.88 INFO::Writing filtered data to file /tmp/RtmpdBrYAP/maaslin3_parallel_test_3c65854af5e5bf/features/filtered_data.tsv
2026-04-27 01:06:08.88 INFO::Running selected transform method: LOG
2026-04-27 01:06:08.88 INFO::Writing normalized, filtered, transformed data to file /tmp/RtmpdBrYAP/maaslin3_parallel_test_3c65854af5e5bf/features/data_transformed.tsv
2026-04-27 01:06:08.88 INFO::Applying z-score to standardize continuous metadata
2026-04-27 01:06:08.89 INFO::Running the linear model component
2026-04-27 01:06:10.25 INFO::Counting total values for each feature
2026-04-27 01:06:10.26 INFO::Running the logistic model component
2026-04-27 01:06:10.38 INFO::Counting total values for each feature
2026-04-27 01:06:10.40 INFO::Creating fits folder
2026-04-27 01:06:10.40 INFO::Writing residuals to file /tmp/RtmpdBrYAP/maaslin3_parallel_test_3c65854af5e5bf/fits/residuals_linear.rds
2026-04-27 01:06:10.40 INFO::Writing fitted values to file /tmp/RtmpdBrYAP/maaslin3_parallel_test_3c65854af5e5bf/fits/fitted_linear.rds
2026-04-27 01:06:10.40 INFO::Writing residuals to file /tmp/RtmpdBrYAP/maaslin3_parallel_test_3c65854af5e5bf/fits/residuals_logistic.rds
2026-04-27 01:06:10.41 INFO::Writing fitted values to file /tmp/RtmpdBrYAP/maaslin3_parallel_test_3c65854af5e5bf/fits/fitted_logistic.rds
2026-04-27 01:06:10.41 INFO::Writing all the results to file (ordered 
            by increasing individual q-values): /tmp/RtmpdBrYAP/maaslin3_parallel_test_3c65854af5e5bf/all_results.tsv
2026-04-27 01:06:10.41 INFO::Writing the significant results without errors (those which have joint q-values less than or equal to the threshold of 0.100000 ) to file (ordered by increasing individual q-values): /tmp/RtmpdBrYAP/maaslin3_parallel_test_3c65854af5e5bf/significant_results.tsv
2026-04-27 01:06:11.15 INFO::Running the linear model component
2026-04-27 01:06:11.16 INFO::Fitting model to feature number 1, a
2026-04-27 01:06:11.17 INFO::Fitting model to feature number 2, b
2026-04-27 01:06:11.17 INFO::Fitting model to feature number 3, c
2026-04-27 01:06:11.18 INFO::Counting total values for each feature
2026-04-27 01:06:11.18 INFO::Running the logistic model component
2026-04-27 01:06:11.19 INFO::Fitting model to feature number 1, a
2026-04-27 01:06:11.20 INFO::Fitting model to feature number 2, b
2026-04-27 01:06:11.20 INFO::Fitting model to feature number 3, c
2026-04-27 01:06:11.21 INFO::Counting total values for each feature
2026-04-27 01:06:11.21 INFO::Re-running abundances for warn_prevalence
2026-04-27 01:06:11.21 INFO::Running selected normalization method: TSS
2026-04-27 01:06:11.22 INFO::Running selected transform method: LOG
2026-04-27 01:06:11.23 INFO::Fitting model to feature number 1, a
2026-04-27 01:06:11.23 INFO::Fitting model to feature number 2, b
2026-04-27 01:06:11.24 INFO::Fitting model to feature number 3, c
2026-04-27 01:06:11.66 INFO::Started 2 mirai daemons via `cores` argument
2026-04-27 01:06:11.66 INFO::Running the linear model component
2026-04-27 01:06:13.07 INFO::Counting total values for each feature
2026-04-27 01:06:13.07 INFO::Running the logistic model component
2026-04-27 01:06:13.16 INFO::Counting total values for each feature
2026-04-27 01:06:13.16 INFO::Re-running abundances for warn_prevalence
2026-04-27 01:06:13.16 INFO::Running selected normalization method: TSS
2026-04-27 01:06:13.17 INFO::Running selected transform method: LOG
2026-04-27 01:06:13.45 INFO::Running the linear model component
2026-04-27 01:06:13.46 INFO::Fitting model to feature number 1, a
2026-04-27 01:06:13.46 INFO::Fitting model to feature number 2, b
2026-04-27 01:06:13.47 INFO::Counting total values for each feature
2026-04-27 01:06:13.47 INFO::Running the logistic model component
2026-04-27 01:06:13.48 INFO::Fitting model to feature number 1, a
2026-04-27 01:06:13.49 INFO::Fitting model to feature number 2, b
2026-04-27 01:06:13.50 INFO::Counting total values for each feature
2026-04-27 01:06:13.50 INFO::Re-running abundances for warn_prevalence
2026-04-27 01:06:13.50 INFO::Running selected normalization method: TSS
2026-04-27 01:06:13.50 INFO::Running selected transform method: LOG
2026-04-27 01:06:13.51 INFO::Fitting model to feature number 1, a
2026-04-27 01:06:13.52 INFO::Fitting model to feature number 2, b
2026-04-27 01:06:13.53 INFO::Creating output folder
2026-04-27 01:06:13.53 INFO::Creating output figures folder
2026-04-27 01:06:13.53 INFO::Writing summary plot of significant
                        results to file: /tmp/RtmpdBrYAP/file3c658549b95a7f/figures/summary_plot.pdf
2026-04-27 01:06:15.89 INFO::Writing association plots (one for each significant association) to output folder: /tmp/RtmpdBrYAP/file3c658549b95a7f/figures
2026-04-27 01:06:15.89 INFO::Plotting associations from most to least significant, grouped by metadata
2026-04-27 01:06:15.90 INFO::Creating scatter plot for continuous 
                        data (linear), var1 vs b
2026-04-27 01:06:16.54 INFO::Creating boxplot for continuous data (logistic), var1 vs a
2026-04-27 01:06:18.51 INFO::Running the linear model component
2026-04-27 01:06:18.53 INFO::Fitting model to feature number 1, a
2026-04-27 01:06:18.60 INFO::Fitting model to feature number 2, b
2026-04-27 01:06:18.74 INFO::Counting total values for each feature
2026-04-27 01:06:18.74 INFO::Running the logistic model component
2026-04-27 01:06:18.75 INFO::Fitting model to feature number 1, a
2026-04-27 01:06:18.85 INFO::Fitting model to feature number 2, b
2026-04-27 01:06:18.94 INFO::Counting total values for each feature
2026-04-27 01:06:18.94 INFO::Re-running abundances for warn_prevalence
2026-04-27 01:06:18.95 INFO::Running selected normalization method: TSS
2026-04-27 01:06:18.95 INFO::Running selected transform method: LOG
2026-04-27 01:06:18.96 INFO::Fitting model to feature number 1, a
2026-04-27 01:06:19.03 INFO::Fitting model to feature number 2, b
[ FAIL 0 | WARN 0 | SKIP 1 | PASS 190 ]

══ Skipped tests (1) ═══════════════════════════════════════════════════════════
• empty test (1): 'test_no_dplyr_in_tests.R:4:1'

[ FAIL 0 | WARN 0 | SKIP 1 | PASS 190 ]
> 
> 
> proc.time()
   user  system elapsed 
159.381   2.911 176.960 

Example timings

maaslin3.Rcheck/maaslin3-Ex.timings

nameusersystemelapsed
maaslin317.485 0.67618.148
maaslin_check_arguments0.1170.0010.118
maaslin_check_formula0.1390.0040.144
maaslin_compute_formula0.1560.0050.161
maaslin_contrast_test17.213 0.34817.549
maaslin_filter0.4530.0180.472
maaslin_fit13.697 0.21614.686
maaslin_log_arguments0.1360.0090.146
maaslin_normalize0.2800.0050.285
maaslin_plot_results39.625 0.52140.970
maaslin_plot_results_from_output35.047 0.35235.388
maaslin_process_metadata0.4810.0350.516
maaslin_read_data0.1330.0090.142
maaslin_reorder_data0.1420.0080.150
maaslin_transform0.5810.0220.603
maaslin_write_results13.438 0.20213.630
maaslin_write_results_lefse_format14.554 0.17714.720
preprocess_dna_mtx0.0030.0000.004
preprocess_taxa_mtx0.0040.0000.004