Back to Build/check report for BioC 3.23:   simplified   long
ABCDEFGHIJKLMN[O]PQRSTUVWXYZ

This page was generated on 2026-02-21 11:32 -0500 (Sat, 21 Feb 2026).

HostnameOSArch (*)R versionInstalled pkgs
nebbiolo1Linux (Ubuntu 24.04.3 LTS)x86_64R Under development (unstable) (2026-01-15 r89304) -- "Unsuffered Consequences" 4871
Click on any hostname to see more info about the system (e.g. compilers)      (*) as reported by 'uname -p', except on Windows and Mac OS X

Package 1523/2354HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
ORFik 1.31.3  (landing page)
Haakon Tjeldnes
Snapshot Date: 2026-02-20 13:40 -0500 (Fri, 20 Feb 2026)
git_url: https://git.bioconductor.org/packages/ORFik
git_branch: devel
git_last_commit: e7639c3
git_last_commit_date: 2025-12-19 08:49:54 -0500 (Fri, 19 Dec 2025)
nebbiolo1Linux (Ubuntu 24.04.3 LTS) / x86_64  OK    OK    ERROR  
See other builds for ORFik in R Universe.


CHECK results for ORFik on nebbiolo1

To the developers/maintainers of the ORFik package:
- Allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/ORFik.git to reflect on this report. See Troubleshooting Build Report for more information.
- Use the following Renviron settings to reproduce errors and warnings.
- If 'R CMD check' started to fail recently on the Linux builder(s) over a missing dependency, add the missing dependency to 'Suggests:' in your DESCRIPTION file. See Renviron.bioc for more information.

raw results


Summary

Package: ORFik
Version: 1.31.3
Command: /home/biocbuild/bbs-3.23-bioc/R/bin/R CMD check --install=check:ORFik.install-out.txt --library=/home/biocbuild/bbs-3.23-bioc/R/site-library --timings ORFik_1.31.3.tar.gz
StartedAt: 2026-02-21 02:21:22 -0500 (Sat, 21 Feb 2026)
EndedAt: 2026-02-21 02:42:54 -0500 (Sat, 21 Feb 2026)
EllapsedTime: 1291.6 seconds
RetCode: 1
Status:   ERROR  
CheckDir: ORFik.Rcheck
Warnings: NA

Command output

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.23-bioc/R/bin/R CMD check --install=check:ORFik.install-out.txt --library=/home/biocbuild/bbs-3.23-bioc/R/site-library --timings ORFik_1.31.3.tar.gz
###
##############################################################################
##############################################################################


* using log directory ‘/home/biocbuild/bbs-3.23-bioc/meat/ORFik.Rcheck’
* using R Under development (unstable) (2026-01-15 r89304)
* using platform: x86_64-pc-linux-gnu
* R was compiled by
    gcc (Ubuntu 13.3.0-6ubuntu2~24.04) 13.3.0
    GNU Fortran (Ubuntu 13.3.0-6ubuntu2~24.04) 13.3.0
* running under: Ubuntu 24.04.3 LTS
* using session charset: UTF-8
* checking for file ‘ORFik/DESCRIPTION’ ... OK
* checking extension type ... Package
* this is package ‘ORFik’ version ‘1.31.3’
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... INFO
Imports includes 29 non-default packages.
Importing from so many packages makes the package vulnerable to any of
them becoming unavailable.  Move as many as possible to Suggests and
use conditionally.
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... NOTE
Found the following non-portable file path:
  ORFik/inst/extdata/test_processing/contaminants_depletion/LOGS/contaminants_output_template_Log.final.out

Tarballs are only required to store paths of up to 100 bytes and cannot
store those of more than 256 bytes, with restrictions including to 100
bytes for the final component.
See section ‘Package structure’ in the ‘Writing R Extensions’ manual.
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘ORFik’ can be installed ... OK
* used C++ compiler: ‘g++ (Ubuntu 13.3.0-6ubuntu2~24.04) 13.3.0’
* checking installed package size ... INFO
  installed size is  5.6Mb
  sub-directories of 1Mb or more:
    R      1.2Mb
    help   1.1Mb
    libs   1.7Mb
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... NOTE
License stub is invalid DCF.
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking code files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... NOTE
Warning: no function found corresponding to methods exports from ‘ORFik’ for: ‘organism’

A namespace must be able to be loaded with just the base namespace
loaded: otherwise if the namespace gets loaded by a saved object, the
session will be unable to start.

Probably some imports need to be declared in the NAMESPACE file.
* checking whether the namespace can be unloaded cleanly ... OK
* checking loading without being on the library search path ... OK
* checking whether startup messages can be suppressed ... OK
* checking dependencies in R code ... NOTE
Unexported objects imported by ':::' calls:
  ‘GenomicFeatures:::.merge_seqinfo_and_infer_missing_seqlengths’
  ‘IRanges:::regroupBySupergroup’ ‘S4Vectors:::normarg_mcols’
  ‘biomartr:::getENSEMBL.Seq’ ‘biomartr:::getENSEMBL.gtf’
  ‘qs2:::check_TBB’
  See the note in ?`:::` about the use of this operator.
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... NOTE
DEG.plot.static: no visible binding for global variable ‘Regulation’
DEG.plot.static: no visible binding for global variable ‘meanCounts’
DEG.plot.static: no visible binding for global variable ‘LFC’
DEG_gorilla: no visible binding for global variable ‘contrast’
DEG_gorilla: no visible binding for global variable ‘Regulation’
DEG_gorilla: no visible binding for global variable
  ‘external_gene_name’
DEG_gorilla_local_load_data: no visible binding for global variable
  ‘analysis’
DEG_gorilla_local_load_data: no visible binding for global variable
  ‘.id’
DEG_gorilla_local_load_data: no visible binding for global variable
  ‘p.adjust’
DEG_gorilla_local_load_data: no visible binding for global variable
  ‘FDR q-value’
DEG_gorilla_local_load_data: no visible binding for global variable
  ‘Enrichment’
DEG_gorilla_local_load_data: no visible binding for global variable
  ‘GeneRatio’
DEG_gorilla_local_load_data: no visible binding for global variable ‘b’
DEG_gorilla_local_load_data: no visible binding for global variable ‘n’
DEG_gorilla_plot: no visible binding for global variable ‘p.adjust’
DEG_gorilla_plot: no visible binding for global variable ‘GeneRatio’
DEG_gorilla_plot: no visible binding for global variable ‘Enrichment’
DEG_gorilla_plot: no visible global function definition for ‘head’
DEG_gorilla_plot: no visible global function definition for ‘.’
DEG_gorilla_plot: no visible binding for global variable ‘analysis’
DEG_gorilla_plot: no visible binding for global variable ‘go_category’
DEG_gorilla_plot: no visible binding for global variable ‘Description’
DEG_gorilla_plot: no visible binding for global variable ‘b’
DEG_model_simple: no visible binding for global variable ‘id’
DEG_model_simple: no visible binding for global variable ‘contrast’
DTEG.plot: no visible binding for global variable ‘Regulation’
DTEG.plot: no visible binding for global variable ‘rna.lfc’
DTEG.plot: no visible binding for global variable ‘rfp.lfc’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘te.sign’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘rfp.sign’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘rna.sign’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘Regulation’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘all_models_sign’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘te.lfc’
DTEG_add_regulation_categories: no visible binding for global variable
  ‘rna.lfc’
DTEG_pair_results: no visible binding for global variable ‘rna.sign’
DTEG_pair_results: no visible binding for global variable ‘rna.padj’
DTEG_pair_results: no visible binding for global variable ‘rfp.sign’
DTEG_pair_results: no visible binding for global variable ‘rfp.padj’
DTEG_pair_results: no visible binding for global variable ‘te.sign’
DTEG_pair_results: no visible binding for global variable ‘te.padj’
DTEG_pair_results: no visible binding for global variable
  ‘all_models_sign’
QCstats.plot: no visible binding for global variable ‘variable’
QCstats.plot: no visible binding for global variable ‘sample_total’
QCstats.plot: no visible binding for global variable ‘value’
QCstats.plot: no visible global function definition for ‘.’
QCstats.plot: no visible binding for global variable ‘sample_id’
QCstats.plot: no visible binding for global variable ‘percentage’
QCstats.plot: no visible binding for global variable
  ‘perc_of_counts_per_sample’
QCstats.plot: no visible binding for global variable ‘read length’
RiboQC.plot: no visible binding for global variable ‘variable’
RiboQC.plot: no visible binding for global variable ‘sample_total’
RiboQC.plot: no visible binding for global variable ‘value’
RiboQC.plot: no visible global function definition for ‘.’
RiboQC.plot: no visible binding for global variable ‘sample_id’
RiboQC.plot: no visible binding for global variable ‘percentage’
RiboQC.plot: no visible binding for global variable ‘percent’
RiboQC.plot: no visible binding for global variable ‘frame’
STAR.multiQC_plot: no visible binding for global variable ‘value’
STAR.multiQC_plot: no visible binding for global variable ‘sample_id’
TOP.Motif.ecdf: no visible binding for global variable ‘seq1’
TOP.Motif.ecdf: no visible binding for global variable ‘TOP’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘percentage_mrna_aligned’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘mRNA’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘percentage_tx_aligned’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘Transcript’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘ratio_cds_mrna’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘CDS’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘ratio_cds_leader’
alignmentFeatureStatistics : <anonymous>: no visible binding for global
  variable ‘LEADERS’
allFeaturesHelper: no visible binding for global variable ‘te’
allFeaturesHelper: no visible binding for global variable ‘fpkmRFP’
allFeaturesHelper: no visible binding for global variable ‘fpkmRNA’
allFeaturesHelper: no visible binding for global variable ‘countRFP’
allFeaturesHelper: no visible binding for global variable ‘entropyRFP’
allFeaturesHelper: no visible binding for global variable
  ‘disengagementScores’
allFeaturesHelper: no visible binding for global variable ‘RRS’
allFeaturesHelper: no visible binding for global variable ‘RSS’
allFeaturesHelper: no visible binding for global variable ‘ORFScores’
allFeaturesHelper: no visible binding for global variable ‘ioScore’
allFeaturesHelper: no visible binding for global variable
  ‘startCodonCoverage’
allFeaturesHelper: no visible binding for global variable
  ‘startRegionRelative’
allFeaturesHelper: no visible binding for global variable ‘kozak’
allFeaturesHelper: no visible binding for global variable ‘StartCodons’
allFeaturesHelper: no visible binding for global variable ‘StopCodons’
allFeaturesHelper: no visible binding for global variable
  ‘fractionLengths’
allFeaturesHelper: no visible binding for global variable ‘distORFCDS’
allFeaturesHelper: no visible binding for global variable ‘inFrameCDS’
allFeaturesHelper: no visible binding for global variable
  ‘isOverlappingCds’
allFeaturesHelper: no visible binding for global variable ‘rankInTx’
appendZeroes: no visible binding for global variable ‘frame’
appendZeroes: no visible binding for global variable ‘position’
appendZeroes: no visible binding for global variable ‘count’
append_gene_symbols: no visible binding for global variable
  ‘id_original’
append_gene_symbols: no visible binding for global variable ‘id’
append_gene_symbols: no visible binding for global variable
  ‘external_gene_name’
append_gene_symbols: no visible binding for global variable ‘label’
artificial.orfs: no visible binding for global variable ‘random’
artificial.orfs: no visible binding for global variable ‘pick’
artificial.orfs: no visible global function definition for ‘.’
bamLoadCollapsedScores: no visible global function definition for
  ‘bamTag<-’
bamLoadCollapsedScores: no visible global function definition for
  ‘bamWhat<-’
browseSRA: no visible global function definition for ‘browseURL’
cellTypeNames: missing arguments not allowed in calls to ‘c’
codonSumsPerGroup: no visible binding for global variable ‘genes’
codon_usage: no visible binding for global variable ‘merged’
codon_usage: no visible binding for global variable ‘AA’
codon_usage: no visible binding for global variable ‘codon’
codon_usage_plot: no visible binding for global variable ‘seqs’
collapse.by.scores: no visible global function definition for ‘.’
collapse.fastq.internal: no visible binding for global variable ‘N’
cor_plot: no visible binding for global variable ‘Var2’
cor_plot: no visible binding for global variable ‘Var1’
cor_plot: no visible binding for global variable ‘Cor’
cor_table: no visible binding for global variable ‘Var1’
cor_table: no visible binding for global variable ‘Var2’
cor_table: no visible binding for global variable ‘Cor’
coverageByTranscriptFST: no visible binding for global variable
  ‘file_forward’
coverageByTranscriptFST: no visible binding for global variable
  ‘file_reverse’
coverageByTranscriptFST : <anonymous>: no visible binding for global
  variable ‘chr’
coverageByTranscriptFST : <anonymous>: no visible binding for global
  variable ‘query_id’
coverageByTranscriptFST : <anonymous>: no visible global function
  definition for ‘foverlaps’
coverageByTranscriptFST : <anonymous>: no visible binding for global
  variable ‘start_segment’
coverageByTranscriptFST : <anonymous>: no visible binding for global
  variable ‘i.start’
coverageByTranscriptFST : <anonymous>: no visible binding for global
  variable ‘end_segment’
coverageByTranscriptFST : <anonymous>: no visible binding for global
  variable ‘i.end’
coverageByTranscriptFST : <anonymous>: no visible binding for global
  variable ‘file_forward’
coverageByTranscriptFST : <anonymous>: no visible binding for global
  variable ‘file_reverse’
coverageByTranscriptFST : <anonymous>: no visible global function
  definition for ‘.’
coverageHeatMap: no visible binding for global variable ‘position’
coverageHeatMap: no visible binding for global variable ‘fraction’
coveragePerORFStatistics: no visible global function definition for ‘.’
coveragePerORFStatistics: no visible binding for global variable
  ‘count’
coveragePerORFStatistics: no visible binding for global variable
  ‘genes’
coverageScorings: no visible binding for global variable ‘count’
coverageScorings: no visible binding for global variable ‘zscore’
coverageScorings: no visible binding for global variable ‘windowMean’
coverageScorings: no visible binding for global variable ‘windowSD’
coverageScorings: no visible global function definition for ‘.’
coverageScorings: no visible binding for global variable ‘modzscore’
coverageScorings: no visible binding for global variable ‘windowMedian’
coverageScorings: no visible binding for global variable ‘windowMAD’
coverageScorings: no visible binding for global variable ‘gene_sum’
coverageScorings: no visible binding for global variable ‘fraction’
coverage_random_access_file: no visible binding for global variable
  ‘count’
coverage_random_access_file: no visible binding for global variable
  ‘genes’
coverage_random_access_file: no visible binding for global variable
  ‘position’
coverage_random_access_file: no visible binding for global variable
  ‘frame’
coverage_to_dt: no visible binding for global variable ‘frame’
coverage_to_dt: no visible binding for global variable ‘genes’
coverage_to_dt: no visible binding for global variable ‘position’
detectRibosomeShifts: no visible global function definition for ‘.’
detectRibosomeShifts: no visible binding for global variable ‘size’
detectRibosomeShifts: no visible binding for global variable ‘fraction’
detectRibosomeShifts: no visible binding for global variable ‘pShifted’
detectRibosomeShifts: no visible binding for global variable
  ‘sum.count’
detectRibosomeShifts: no visible binding for global variable ‘count’
detectRibosomeShifts: no visible binding for global variable ‘genes’
detectRibosomeShifts: no visible binding for global variable
  ‘frac.score’
detect_ribo_orfs: no visible global function definition for ‘organism’
download_gene_homologues: no visible binding for global variable
  ‘species’
entropy: no visible binding for global variable ‘Hx’
entropy: no visible binding for global variable ‘codonSums’
entropy: no visible global function definition for ‘.’
entropy: no visible binding for global variable ‘genes’
experiment_parse_list_info: no visible binding for global variable
  ‘index’
fetch_xml_attributes: no visible binding for global variable
  ‘..to_keep’
filterExtremePeakGenes: no visible binding for global variable ‘count’
filterExtremePeakGenes: no visible binding for global variable
  ‘median_per_gene’
filterExtremePeakGenes: no visible binding for global variable ‘genes’
filterExtremePeakGenes: no visible global function definition for ‘.’
filterTranscripts: no visible binding for global variable ‘utr5_len’
filterTranscripts: no visible binding for global variable ‘utr3_len’
filter_empty_runs: no visible binding for global variable ‘spots’
findNGSPairs: no visible global function definition for ‘.’
findNGSPairs: no visible binding for global variable ‘forward’
findPeaksPerGene: no visible binding for global variable ‘sum_per_gene’
findPeaksPerGene: no visible binding for global variable ‘count’
findPeaksPerGene: no visible binding for global variable ‘genes’
findPeaksPerGene: no visible binding for global variable
  ‘mean_per_gene’
findPeaksPerGene: no visible binding for global variable
  ‘median_per_gene’
findPeaksPerGene: no visible binding for global variable ‘sd_per_gene’
findPeaksPerGene: no visible binding for global variable ‘mad_per_gene’
findPeaksPerGene: no visible binding for global variable ‘zscore’
findPeaksPerGene: no visible binding for global variable ‘modzscore’
findPeaksPerGene: no visible binding for global variable ‘gene_id’
find_url_ebi_safe: no visible binding for global variable
  ‘run_accession’
floss: no visible binding for global variable ‘ORFGrouping’
floss: no visible binding for global variable ‘widths’
floss: no visible global function definition for ‘.’
floss: no visible binding for global variable ‘CDSGrouping’
floss: no visible binding for global variable ‘fraction.x’
floss: no visible binding for global variable ‘fraction.y’
gSort: no visible binding for global variable ‘grnames’
geneToSymbol: no visible global function definition for ‘organism’
geneToSymbol: no visible binding for global variable ‘ensembl_gene_id’
geneToSymbol: no visible binding for global variable
  ‘external_gene_name’
geneToSymbol: no visible binding for global variable
  ‘ensembl_transcript_id’
geneToSymbol: no visible binding for global variable ‘uniprotswissprot’
getNGenesCoverage: no visible global function definition for ‘.’
getNGenesCoverage: no visible binding for global variable ‘genes’
getNGenesCoverage: no visible binding for global variable ‘fraction’
get_phix_genome: no visible binding for global variable ‘phix.url’
go_analaysis_gorilla: no visible global function definition for
  ‘browseURL’
grl_has_any_valid_lengths: no visible binding for global variable ‘grl’
initiationScore: no visible global function definition for ‘.’
initiationScore: no visible binding for global variable ‘dif’
initiationScore: no visible binding for global variable ‘fraction’
initiationScore: no visible binding for global variable ‘genes’
initiationScore: no visible binding for global variable ‘difPer’
install.sratoolkit: no visible binding for global variable ‘osVersion’
isPeriodic: no visible binding for global variable ‘spec’
kozakHeatmap: no visible global function definition for ‘.’
kozakHeatmap: no visible binding for global variable ‘variable’
kozakHeatmap: no visible binding for global variable ‘value’
kozakHeatmap: no visible binding for global variable
  ‘count_seq_pos_with_count’
kozakHeatmap: no visible binding for global variable ‘median_score’
kozak_IR_ranking: no visible global function definition for ‘.’
kozak_IR_ranking: no visible binding for global variable ‘IR’
kozak_IR_ranking: no visible binding for global variable
  ‘upstream_kozak_strength’
kozak_IR_ranking: no visible binding for global variable ‘count’
kozak_IR_ranking: no visible binding for global variable ‘mean_IR’
list.genomes: no visible binding for global variable ‘STAR_index’
longestORFs: no visible global function definition for ‘.’
metaWindow: no visible binding for global variable ‘position’
metaWindow: no visible binding for global variable ‘frame’
metadata.autnaming: no visible binding for global variable
  ‘LIBRARYTYPE’
metadata.autnaming: no visible binding for global variable
  ‘LibraryStrategy’
ofst_merge_internal : <anonymous>: no visible global function
  definition for ‘.’
orfFrameDistributions : <anonymous>: no visible binding for global
  variable ‘fraction’
orfFrameDistributions: no visible binding for global variable ‘percent’
orfFrameDistributions: no visible binding for global variable
  ‘fraction’
orfFrameDistributions: no visible binding for global variable
  ‘percent_length’
orfFrameDistributions: no visible global function definition for ‘.’
orfFrameDistributions: no visible binding for global variable
  ‘best_frame’
orfScore: no visible binding for global variable ‘genes’
orfScore: no visible binding for global variable ‘frame’
orfScore: no visible binding for global variable ‘frame_one_RP’
orfScore: no visible binding for global variable ‘frame_two_RP’
pSitePlot: no visible binding for global variable ‘count’
pSitePlot: no visible binding for global variable ‘frame’
pSitePlot: no visible binding for global variable ‘position’
readLengthTable: no visible binding for global variable
  ‘counts_per_sample’
readLengthTable: no visible binding for global variable ‘sample_id’
readLengthTable: no visible binding for global variable
  ‘perc_of_counts_per_sample’
regionPerReadLength : <anonymous>: no visible binding for global
  variable ‘fraction’
remakeTxdbExonIds: no visible global function definition for ‘.’
remakeTxdbExonIds: no visible binding for global variable ‘chr’
removeTxdbExons: no visible binding for global variable ‘exon_rank’
removeTxdbExons: no visible binding for global variable ‘ranks’
riboORFsFolder: no visible global function definition for ‘organism’
ribo_fft: no visible binding for global variable ‘fraction’
ribo_fft_plot: no visible binding for global variable ‘periods’
ribo_fft_plot: no visible binding for global variable ‘amplitude’
rnaNormalize: no visible binding for global variable ‘genes’
rnaNormalize: no visible binding for global variable ‘feature’
sample_info_append_SRA: no visible binding for global variable ‘GEO’
scaledWindowPositions: no visible binding for global variable
  ‘scalingFactor’
scaledWindowPositions: no visible binding for global variable ‘genes’
scaledWindowPositions: no visible binding for global variable
  ‘position’
scaledWindowPositions: no visible global function definition for ‘.’
scoreSummarizedExperiment: no visible global function definition for
  ‘rowSums2’
seq_usage: no visible binding for global variable ‘variable’
seq_usage: no visible binding for global variable ‘codon_sum’
seq_usage: no visible global function definition for ‘.’
seq_usage: no visible binding for global variable ‘gene_sum’
seq_usage: no visible binding for global variable
  ‘N_AA_of_type_per_gene’
seq_usage: no visible binding for global variable ‘as_prob_normalized’
seq_usage: no visible binding for global variable ‘N_total’
seq_usage: no visible binding for global variable ‘N’
seq_usage: no visible binding for global variable ‘mean_txNorm’
seq_usage: no visible binding for global variable ‘sum_txNorm’
seq_usage: no visible binding for global variable ‘dispersion’
seq_usage: no visible binding for global variable ‘dispersion_txNorm’
seq_usage: no visible binding for global variable ‘var_txNorm’
seq_usage: no visible binding for global variable ‘mean_percentage’
seq_usage: no visible binding for global variable ‘mean_txNorm_prob’
seq_usage: no visible binding for global variable
  ‘mean_txNorm_percentage’
seq_usage: no visible binding for global variable
  ‘relative_to_max_score’
shiftPlots : <anonymous>: no visible binding for global variable
  ‘frame’
shiftPlots : <anonymous>: no visible binding for global variable
  ‘position’
te.plot: no visible global function definition for ‘rowMin’
te.plot: no visible binding for global variable ‘variable’
te.plot: no visible binding for global variable ‘LFC_TE’
te.plot: no visible binding for global variable ‘rfp_log2’
te.plot: no visible binding for global variable ‘rna_log2’
te.plot: no visible binding for global variable ‘rna_log10’
te.table: no visible global function definition for ‘rowMin’
te.table: no visible binding for global variable ‘variable’
te.table: no visible binding for global variable ‘TE_log2’
te.table: no visible binding for global variable ‘rfp_log2’
te.table: no visible binding for global variable ‘rna_log2’
te_rna.plot: no visible binding for global variable ‘subtitle’
te_rna.plot: no visible binding for global variable ‘rna_log10’
te_rna.plot: no visible binding for global variable ‘TE_log2’
topMotif: no visible binding for global variable ‘seq1’
topMotif: no visible binding for global variable ‘seq2’
topMotif: no visible binding for global variable ‘seq3’
topMotif: no visible binding for global variable ‘seq4’
topMotif: no visible binding for global variable ‘seq5’
transcriptWindow: no visible binding for global variable ‘fractions’
transcriptWindow: no visible binding for global variable ‘feature’
unlistToExtremities: no visible global function definition for ‘.’
unlistToExtremities: no visible binding for global variable ‘group’
unlistToExtremities: no visible binding for global variable ‘NAMES’
widthPerGroup: no visible global function definition for ‘.’
widthPerGroup: no visible binding for global variable ‘widths’
windowCoveragePlot: no visible binding for global variable ‘feature’
windowCoveragePlot: no visible binding for global variable ‘fraction’
windowCoveragePlot: no visible binding for global variable
  ‘fraction_min’
windowCoveragePlot: no visible binding for global variable ‘position’
as.character,GRangesList: no visible global function definition for
  ‘unstrsplit’
collapseDuplicatedReads,GAlignmentPairs: no visible global function
  definition for ‘.’
collapseDuplicatedReads,GAlignmentPairs: no visible binding for global
  variable ‘start1’
collapseDuplicatedReads,GAlignmentPairs: no visible binding for global
  variable ‘start2’
collapseDuplicatedReads,GAlignmentPairs: no visible binding for global
  variable ‘cigar1’
collapseDuplicatedReads,GAlignmentPairs: no visible binding for global
  variable ‘cigar2’
collapseDuplicatedReads,GAlignments: no visible global function
  definition for ‘.’
collapseDuplicatedReads,GRanges: no visible binding for global variable
  ‘size’
collapseDuplicatedReads,GRanges: no visible global function definition
  for ‘.’
collapseDuplicatedReads,data.table: no visible global function
  definition for ‘.’
design,experiment: no visible binding for global variable ‘..formula’
seqlevels,experiment: no visible binding for global variable ‘df’
show,covRleList: no visible global function definition for ‘head’
show,experiment: no visible global function definition for ‘organism’
Undefined global functions or variables:
  . ..formula ..to_keep .id AA CDS CDSGrouping Cor Description
  Enrichment FDR q-value GEO GeneRatio Hx IR LEADERS LFC LFC_TE
  LIBRARYTYPE LibraryStrategy N NAMES N_AA_of_type_per_gene N_total
  ORFGrouping ORFScores RRS RSS Regulation STAR_index StartCodons
  StopCodons TE_log2 TOP Transcript Var1 Var2 all_models_sign amplitude
  analysis as_prob_normalized b bamTag<- bamWhat<- best_frame browseURL
  chr cigar1 cigar2 codon codonSums codon_sum contrast count countRFP
  count_seq_pos_with_count counts_per_sample df dif difPer
  disengagementScores dispersion dispersion_txNorm distORFCDS
  end_segment ensembl_gene_id ensembl_transcript_id entropyRFP
  exon_rank external_gene_name feature file_forward file_reverse
  forward foverlaps fpkmRFP fpkmRNA frac.score fraction fraction.x
  fraction.y fractionLengths fraction_min fractions frame frame_one_RP
  frame_two_RP gene_id gene_sum genes go_category grl grnames group
  head i.end i.start id id_original inFrameCDS index ioScore
  isOverlappingCds kozak label mRNA mad_per_gene meanCounts mean_IR
  mean_per_gene mean_percentage mean_txNorm mean_txNorm_percentage
  mean_txNorm_prob median_per_gene median_score merged modzscore n
  organism osVersion p.adjust pShifted perc_of_counts_per_sample
  percent percent_length percentage percentage_mrna_aligned
  percentage_tx_aligned periods phix.url pick position query_id random
  rankInTx ranks ratio_cds_leader ratio_cds_mrna read length
  relative_to_max_score rfp.lfc rfp.padj rfp.sign rfp_log2 rna.lfc
  rna.padj rna.sign rna_log10 rna_log2 rowMin rowSums2 run_accession
  sample_id sample_total scalingFactor sd_per_gene seq1 seq2 seq3 seq4
  seq5 seqs size spec species spots start1 start2 startCodonCoverage
  startRegionRelative start_segment subtitle sum.count sum_per_gene
  sum_txNorm te te.lfc te.padj te.sign uniprotswissprot unstrsplit
  upstream_kozak_strength utr3_len utr5_len value var_txNorm variable
  widths windowMAD windowMean windowMedian windowSD zscore
Consider adding
  importFrom("base", "length")
  importFrom("graphics", "frame")
  importFrom("stats", "df", "p.adjust")
  importFrom("utils", "browseURL", "head", "osVersion")
to your NAMESPACE file.
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... NOTE
Found the following Rd file(s) with Rd \link{} targets missing package
anchors:
  DEG.analysis.Rd: estimateDispersions
  DEG_model.Rd: estimateDispersions
  DTEG.analysis.Rd: SummarizedExperiment, estimateDispersions
  ORFikQC.Rd: SummarizedExperiment
  QCreport.Rd: SummarizedExperiment
  allFeaturesHelper.Rd: GRangesList, GAlignments, GRanges, FaFile
  as.character-GRangesList-method.Rd: GRangesList
  asTX.Rd: GRangesList
  assignFirstExonsStartSite.Rd: GRangesList
  assignLastExonsStopSite.Rd: GRangesList
  bedToGR.Rd: GRanges
  codonSumsPerGroup.Rd: GRangesList, GAlignments, GRanges
  codon_usage_exp.Rd: FaFile
  computeFeatures.Rd: GRangesList, GAlignments, GRanges, FaFile
  computeFeaturesCage.Rd: GRangesList, GAlignments, GRanges, FaFile
  coverageByTranscriptC.Rd: coverageByTranscript, GRangesList
  coverageByTranscriptW.Rd: coverageByTranscript, GRanges, GAlignments,
    GRangesList
  coveragePerTiling.Rd: GRangesList, GAlignments, GRanges
  detectRibosomeShifts.Rd: GAlignments
  disengagementScore.Rd: GRangesList, TxDb
  distToCds.Rd: GRangesList
  distToTSS.Rd: GRangesList
  downstreamFromPerGroup.Rd: GRangesList
  downstreamOfPerGroup.Rd: GRangesList
  entropy.Rd: GRangesList, GRanges, GAlignments
  experiment-class.Rd: SummarizedExperiment
  export.ofst-GAlignmentPairs-method.Rd: fst-package
  export.ofst-GAlignments-method.Rd: fst-package
  export.ofst-GRanges-method.Rd: fst-package
  export.ofst-data.frame-method.Rd: fst-package
  export.ofst.Rd: fst-package
  extendLeaders.Rd: GRangesList
  extendTrailers.Rd: GRangesList
  filterExtremePeakGenes.Rd: GAlignments, GRanges
  fimport.Rd: seqlevelsStyle, Seqinfo, GAlignments, GRanges
  findFa.Rd: FaFile
  findMapORFs.Rd: GRangesList, FaFile
  findORFs.Rd: IRanges, IRangesList, FaFile
  findPeaksPerGene.Rd: GAlignments, GRanges
  findUORFs.Rd: FaFile
  firstEndPerGroup.Rd: GRangesList
  firstExonPerGroup.Rd: GRangesList
  firstStartPerGroup.Rd: GRangesList
  flankPerGroup.Rd: GRangesList
  floss.Rd: GRangesList, GRanges, GAlignments
  fpkm.Rd: GRangesList, GRanges, GAlignments
  fractionLength.Rd: GRangesList
  fread.bed.Rd: import.bed, seqlevelsStyle, Seqinfo, GRanges
  gSort.Rd: GRangesList
  hasHits.Rd: GRangesList
  heatMapL.Rd: GRangesList
  heatMapRegion.Rd: GRanges, GAlignments
  heatMap_single.Rd: GRangesList, GAlignments, GRanges
  import.ofst.Rd: fst-package, GAlignmentPairs
  initiationScore.Rd: GRangesList, GAlignments
  insideOutsideORF.Rd: GRangesList, TxDb
  kozakSequenceScore.Rd: GRangesList, FaFile
  kozak_IR_ranking.Rd: FaFile
  lastExonEndPerGroup.Rd: GRangesList
  lastExonPerGroup.Rd: GRangesList
  lastExonStartPerGroup.Rd: GRangesList
  loadTxdb.Rd: seqlevelsStyle, Seqinfo
  longestORFs.Rd: GRangesList
  makeExonRanks.Rd: GRangesList
  makeORFNames.Rd: GRangesList
  makeSummarizedExperimentFromBam.Rd: GRangesList, SummarizedExperiment
  mapToGRanges.Rd: GRangesList
  matchNaming.Rd: GRangesList
  matchSeqStyle.Rd: seqlevelsStyle, Seqinfo
  numCodons.Rd: GRangesList
  numExonsPerGroup.Rd: GRangesList
  optimizeReads.Rd: GRangesList
  optimizedTranscriptLengths.Rd: transcriptLengths
  orfID.Rd: GRangesList
  orfScore.Rd: GRangesList, GAlignments, GRanges
  outputLibs.Rd: seqlevelsStyle, Seqinfo
  rankOrder.Rd: GRangesList
  readBam.Rd: readGAlignments, seqlevelsStyle, Seqinfo, GAlignments,
    GAlignmentPairs
  readBigWig.Rd: seqlevelsStyle, Seqinfo, GRanges
  readWig.Rd: seqlevelsStyle, Seqinfo, GRanges
  reduceKeepAttr.Rd: GRangesList, reduce
  regionPerReadLength.Rd: GRangesList, GAlignments, GRanges
  removeMetaCols.Rd: GRangesList
  reverseMinusStrandPerGroup.Rd: GRangesList
  ribo_fft.Rd: GAlignments, GRanges, GRangesList
  ribosomeReleaseScore.Rd: GRangesList, GAlignments, GRanges
  ribosomeStallingScore.Rd: GRangesList
  rnaNormalize.Rd: GRangesList
  scaledWindowPositions.Rd: GRangesList, GAlignments, GRanges
  seqnamesPerGroup.Rd: GRangesList
  shiftFootprints.Rd: GAlignments, GRanges
  sortPerGroup.Rd: sort.GenomicRanges, GRangesList
  splitIn3Tx.Rd: GRangesList
  startCodons.Rd: GRangesList
  startRegion.Rd: GRangesList
  startRegionCoverage.Rd: GRangesList
  startRegionString.Rd: GRangesList, FaFile
  startSites.Rd: GRangesList
  stopCodons.Rd: GRangesList
  stopRegion.Rd: GRangesList
  stopSites.Rd: GRangesList
  strandBool.Rd: GRangesList
  strandPerGroup.Rd: GRangesList
  tile1.Rd: GRangesList
  transcriptWindow.Rd: GRangesList
  transcriptWindowPer.Rd: GRangesList
  translationalEff.Rd: GRangesList, GRanges, GAlignments
  txNames.Rd: GRangesList
  txSeqsFromFa.Rd: GRangesList, FaFile, extractTranscriptSeqs,
    DNAStringSet
  uniqueGroups.Rd: GRangesList
  uniqueOrder.Rd: GRangesList, GRanges
  unlistToExtremities.Rd: GRangesList
  upstreamFromPerGroup.Rd: GRangesList
  upstreamOfPerGroup.Rd: GRangesList
  validSeqlevels.Rd: GRangesList
  widthPerGroup.Rd: GRangesList
  windowPerGroup.Rd: GRangesList
  windowPerReadLength.Rd: GRangesList, GAlignments, GRanges
Please provide package anchors for all Rd \link{} targets not in the
package itself and the base packages.
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking line endings in C/C++/Fortran sources/headers ... OK
* checking compiled code ... INFO
Note: information on .o files is not available
* checking files in ‘vignettes’ ... OK
* checking examples ... OK
Examples with CPU (user + system) or elapsed time > 5s
                               user system elapsed
orfFrameDistributions        68.178 10.641  30.505
orfScore                     27.431  3.555   3.526
makeTxdbFromGenome           24.528  1.001  26.314
codon_usage_exp              16.120  1.796  18.498
STAR.allsteps.multiQC        16.877  0.944  21.734
DEG.analysis                 13.037  0.804  16.033
computeFeatures               8.064  0.793   9.197
pcaExperiment                 6.463  1.401   7.863
shiftPlots                    4.557  2.092   0.758
makeGRangesFromDataFrameFast  6.044  0.488   6.532
codon_usage_plot              5.526  0.925   7.046
detect_ribo_orfs              5.404  0.574   5.524
countTable_regions            4.145  1.606   2.896
ribo_fft_plot                 4.881  0.318   6.185
ribo_fft                      4.589  0.535   6.096
shiftFootprintsByExperiment   2.079  0.194   8.001
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘testthat.R’
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes ... OK
* checking re-building of vignette outputs ... ERROR
Error(s) in re-building vignettes:
--- re-building ‘Annotation_Alignment.Rmd’ using rmarkdown
--- finished re-building ‘Annotation_Alignment.Rmd’

--- re-building ‘Importing_Data.Rmd’ using rmarkdown
--- finished re-building ‘Importing_Data.Rmd’

--- re-building ‘ORFikExperiment.Rmd’ using rmarkdown
The magick package is required to crop "/home/biocbuild/bbs-3.23-bioc/meat/ORFik.Rcheck/vign_test/ORFik/vignettes/ORFikExperiment_files/figure-html/unnamed-chunk-21-1.png" but not available.
The magick package is required to crop "/home/biocbuild/bbs-3.23-bioc/meat/ORFik.Rcheck/vign_test/ORFik/vignettes/ORFikExperiment_files/figure-html/unnamed-chunk-21-2.png" but not available.
--- finished re-building ‘ORFikExperiment.Rmd’

--- re-building ‘ORFikOverview.Rmd’ using rmarkdown
The magick package is required to crop "/home/biocbuild/bbs-3.23-bioc/meat/ORFik.Rcheck/vign_test/ORFik/vignettes/ORFikOverview_files/figure-html/unnamed-chunk-32-1.png" but not available.
The magick package is required to crop "/home/biocbuild/bbs-3.23-bioc/meat/ORFik.Rcheck/vign_test/ORFik/vignettes/ORFikOverview_files/figure-html/unnamed-chunk-36-1.png" but not available.
The magick package is required to crop "/home/biocbuild/bbs-3.23-bioc/meat/ORFik.Rcheck/vign_test/ORFik/vignettes/ORFikOverview_files/figure-html/unnamed-chunk-37-1.png" but not available.
The magick package is required to crop "/home/biocbuild/bbs-3.23-bioc/meat/ORFik.Rcheck/vign_test/ORFik/vignettes/ORFikOverview_files/figure-html/unnamed-chunk-46-1.png" but not available.
The magick package is required to crop "/home/biocbuild/bbs-3.23-bioc/meat/ORFik.Rcheck/vign_test/ORFik/vignettes/ORFikOverview_files/figure-html/unnamed-chunk-51-1.png" but not available.
The magick package is required to crop "/home/biocbuild/bbs-3.23-bioc/meat/ORFik.Rcheck/vign_test/ORFik/vignettes/ORFikOverview_files/figure-html/unnamed-chunk-52-1.png" but not available.
The magick package is required to crop "/home/biocbuild/bbs-3.23-bioc/meat/ORFik.Rcheck/vign_test/ORFik/vignettes/ORFikOverview_files/figure-html/unnamed-chunk-53-1.png" but not available.
The magick package is required to crop "/home/biocbuild/bbs-3.23-bioc/meat/ORFik.Rcheck/vign_test/ORFik/vignettes/ORFikOverview_files/figure-html/unnamed-chunk-54-1.png" but not available.
The magick package is required to crop "/home/biocbuild/bbs-3.23-bioc/meat/ORFik.Rcheck/vign_test/ORFik/vignettes/ORFikOverview_files/figure-html/unnamed-chunk-55-1.png" but not available.
--- finished re-building ‘ORFikOverview.Rmd’

--- re-building ‘Ribo-seq_pipeline-human.Rmd’ using rmarkdown
--- finished re-building ‘Ribo-seq_pipeline-human.Rmd’

--- re-building ‘Ribo-seq_pipeline-yeast.Rmd’ using rmarkdown
--- finished re-building ‘Ribo-seq_pipeline-yeast.Rmd’

--- re-building ‘Working_with_transcripts.Rmd’ using rmarkdown

Quitting from Working_with_transcripts.Rmd:28-42 [unnamed-chunk-1]
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
<error/rlang_error>
Error in `get_genome_fasta()`:
! Genome could not be download, do you have internet.
                 If so, debug to find the reason.
---
Backtrace:
    ▆
 1. └─ORFik::getGenomeAndAnnotation(...)
 2.   └─ORFik:::get_genome_fasta(...)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Error: processing vignette 'Working_with_transcripts.Rmd' failed with diagnostics:
Genome could not be download, do you have internet.
                 If so, debug to find the reason.
--- failed re-building ‘Working_with_transcripts.Rmd’

SUMMARY: processing the following file failed:
  ‘Working_with_transcripts.Rmd’

Error: Vignette re-building failed.
Execution halted

* checking PDF version of manual ... OK
* DONE

Status: 1 ERROR, 6 NOTEs
See
  ‘/home/biocbuild/bbs-3.23-bioc/meat/ORFik.Rcheck/00check.log’
for details.


Installation output

ORFik.Rcheck/00install.out

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.23-bioc/R/bin/R CMD INSTALL ORFik
###
##############################################################################
##############################################################################


* installing to library ‘/home/biocbuild/bbs-3.23-bioc/R/site-library’
* installing *source* package ‘ORFik’ ...
** this is package ‘ORFik’ version ‘1.31.3’
** using staged installation
** libs
using C++ compiler: ‘g++ (Ubuntu 13.3.0-6ubuntu2~24.04) 13.3.0’
g++ -std=gnu++17 -I"/home/biocbuild/bbs-3.23-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.23-bioc/R/site-library/Rcpp/include' -I/usr/local/include    -fpic  -g -O2  -Wall -Werror=format-security  -c RcppExports.cpp -o RcppExports.o
g++ -std=gnu++17 -I"/home/biocbuild/bbs-3.23-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.23-bioc/R/site-library/Rcpp/include' -I/usr/local/include    -fpic  -g -O2  -Wall -Werror=format-security  -c findORFsHelpers.cpp -o findORFsHelpers.o
findORFsHelpers.cpp: In function ‘Rcpp::IntegerMatrix orfs_as_matrix(std::string&, std::string, std::string, int)’:
findORFsHelpers.cpp:214:21: warning: comparison of integer expressions of different signedness: ‘int’ and ‘size_t’ {aka ‘long unsigned int’} [-Wsign-compare]
  214 |   for (int i = 0; i < uorfSize/2; i++) {
      |                   ~~^~~~~~~~~~~~
g++ -std=gnu++17 -I"/home/biocbuild/bbs-3.23-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.23-bioc/R/site-library/Rcpp/include' -I/usr/local/include    -fpic  -g -O2  -Wall -Werror=format-security  -c findOrfs.cpp -o findOrfs.o
g++ -std=gnu++17 -I"/home/biocbuild/bbs-3.23-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.23-bioc/R/site-library/Rcpp/include' -I/usr/local/include    -fpic  -g -O2  -Wall -Werror=format-security  -c findOrfsFasta.cpp -o findOrfsFasta.o
g++ -std=gnu++17 -I"/home/biocbuild/bbs-3.23-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.23-bioc/R/site-library/Rcpp/include' -I/usr/local/include    -fpic  -g -O2  -Wall -Werror=format-security  -c pmapFromTranscripts.cpp -o pmapFromTranscripts.o
pmapFromTranscripts.cpp: In function ‘Rcpp::List pmapFromTranscriptsCPP(const std::vector<int>&, const std::vector<int>&, const std::vector<int>&, const std::vector<int>&, const std::vector<int>&, const char&, bool)’:
pmapFromTranscripts.cpp:151:20: warning: comparison of integer expressions of different signedness: ‘int’ and ‘size_t’ {aka ‘long unsigned int’} [-Wsign-compare]
  151 |   for(auto i = 0;i < xSize; i++){
      |                  ~~^~~~~~~
pmapFromTranscripts.cpp:155:21: warning: comparison of integer expressions of different signedness: ‘int’ and ‘size_t’ {aka ‘long unsigned int’} [-Wsign-compare]
  155 |   for(auto i = 0; i < indexSize; i++){
      |                   ~~^~~~~~~~~~~
pmapFromTranscripts.cpp:161:21: warning: comparison of integer expressions of different signedness: ‘int’ and ‘size_t’ {aka ‘long unsigned int’} [-Wsign-compare]
  161 |   for(auto i = 1; i < indexSize; i++){
      |                   ~~^~~~~~~~~~~
g++ -std=gnu++17 -I"/home/biocbuild/bbs-3.23-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.23-bioc/R/site-library/Rcpp/include' -I/usr/local/include    -fpic  -g -O2  -Wall -Werror=format-security  -c pmapToTranscripts.cpp -o pmapToTranscripts.o
pmapToTranscripts.cpp: In function ‘void pmapToPositive(vi&, const int&, int&, const vi&, const vi&, const vi&, const vi&, const vi&, const vi&, const vi&, const vi&)’:
pmapToTranscripts.cpp:22:7: warning: variable ‘currentWidth’ set but not used [-Wunused-but-set-variable]
   22 |   int currentWidth = 0;
      |       ^~~~~~~~~~~~
pmapToTranscripts.cpp: In function ‘void pmapToNegative(vi&, const int&, int&, const vi&, const vi&, const vi&, const vi&, const vi&, const vi&, const vi&, const vi&)’:
pmapToTranscripts.cpp:68:7: warning: variable ‘currentWidth’ set but not used [-Wunused-but-set-variable]
   68 |   int currentWidth = 0;
      |       ^~~~~~~~~~~~
pmapToTranscripts.cpp: In function ‘Rcpp::List pmapToTranscriptsCPP(const std::vector<int>&, const std::vector<int>&, const std::vector<int>&, const std::vector<int>&, const std::vector<int>&, const char&, const std::vector<int>&)’:
pmapToTranscripts.cpp:126:20: warning: comparison of integer expressions of different signedness: ‘int’ and ‘size_t’ {aka ‘long unsigned int’} [-Wsign-compare]
  126 |   for(auto i = 0;i < xSize; i++){ // Width per exon in x
      |                  ~~^~~~~~~
pmapToTranscripts.cpp:130:21: warning: comparison of integer expressions of different signedness: ‘int’ and ‘size_t’ {aka ‘long unsigned int’} [-Wsign-compare]
  130 |   for(auto i = 0; i < indexSize; i++){ // Width per exon in tx
      |                   ~~^~~~~~~~~~~
pmapToTranscripts.cpp:136:21: warning: comparison of integer expressions of different signedness: ‘int’ and ‘size_t’ {aka ‘long unsigned int’} [-Wsign-compare]
  136 |   for(auto i = 1; i < indexSize; i++){
      |                   ~~^~~~~~~~~~~
pmapToTranscripts.cpp:147:22: warning: comparison of integer expressions of different signedness: ‘int’ and ‘size_t’ {aka ‘long unsigned int’} [-Wsign-compare]
  147 |   for (auto i = 1; i < 2 * xSize; i = i + 2) {
      |                    ~~^~~~~~~~~~~
g++ -std=gnu++17 -shared -L/home/biocbuild/bbs-3.23-bioc/R/lib -L/usr/local/lib -o ORFik.so RcppExports.o findORFsHelpers.o findOrfs.o findOrfsFasta.o pmapFromTranscripts.o pmapToTranscripts.o -L/home/biocbuild/bbs-3.23-bioc/R/lib -lR
installing to /home/biocbuild/bbs-3.23-bioc/R/site-library/00LOCK-ORFik/00new/ORFik/libs
** R
** inst
** byte-compile and prepare package for lazy loading
Creating a new generic function for ‘symbols’ in package ‘ORFik’
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** checking absolute paths in shared objects and dynamic libraries
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (ORFik)

Tests output

ORFik.Rcheck/tests/testthat.Rout


R Under development (unstable) (2026-01-15 r89304) -- "Unsuffered Consequences"
Copyright (C) 2026 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(testthat)
> library(ORFik)
Loading required package: IRanges
Loading required package: BiocGenerics
Loading required package: generics

Attaching package: 'generics'

The following objects are masked from 'package:base':

    as.difftime, as.factor, as.ordered, intersect, is.element, setdiff,
    setequal, union


Attaching package: 'BiocGenerics'

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, is.unsorted, lapply,
    mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int,
    rank, rbind, rownames, sapply, saveRDS, table, tapply, unique,
    unsplit, which.max, which.min

Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following object is masked from 'package:utils':

    findMatches

The following objects are masked from 'package:base':

    I, expand.grid, unname

Loading required package: GenomicRanges
Loading required package: Seqinfo
Loading required package: GenomicAlignments
Loading required package: SummarizedExperiment
Loading required package: MatrixGenerics
Loading required package: matrixStats

Attaching package: 'MatrixGenerics'

The following objects are masked from 'package:matrixStats':

    colAlls, colAnyNAs, colAnys, colAvgsPerRowSet, colCollapse,
    colCounts, colCummaxs, colCummins, colCumprods, colCumsums,
    colDiffs, colIQRDiffs, colIQRs, colLogSumExps, colMadDiffs,
    colMads, colMaxs, colMeans2, colMedians, colMins, colOrderStats,
    colProds, colQuantiles, colRanges, colRanks, colSdDiffs, colSds,
    colSums2, colTabulates, colVarDiffs, colVars, colWeightedMads,
    colWeightedMeans, colWeightedMedians, colWeightedSds,
    colWeightedVars, rowAlls, rowAnyNAs, rowAnys, rowAvgsPerColSet,
    rowCollapse, rowCounts, rowCummaxs, rowCummins, rowCumprods,
    rowCumsums, rowDiffs, rowIQRDiffs, rowIQRs, rowLogSumExps,
    rowMadDiffs, rowMads, rowMaxs, rowMeans2, rowMedians, rowMins,
    rowOrderStats, rowProds, rowQuantiles, rowRanges, rowRanks,
    rowSdDiffs, rowSds, rowSums2, rowTabulates, rowVarDiffs, rowVars,
    rowWeightedMads, rowWeightedMeans, rowWeightedMedians,
    rowWeightedSds, rowWeightedVars

Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.


Attaching package: 'Biobase'

The following object is masked from 'package:MatrixGenerics':

    rowMedians

The following objects are masked from 'package:matrixStats':

    anyMissing, rowMedians

Loading required package: Biostrings
Loading required package: XVector

Attaching package: 'Biostrings'

The following object is masked from 'package:base':

    strsplit

Loading required package: Rsamtools

Attaching package: 'ORFik'

The following object is masked from 'package:graphics':

    symbols

> 
> test_check("ORFik")
[ FAIL 0 | WARN 13 | SKIP 0 | PASS 452 ]

[ FAIL 0 | WARN 13 | SKIP 0 | PASS 452 ]
> 
> proc.time()
   user  system elapsed 
 88.484   4.240  89.945 

Example timings

ORFik.Rcheck/ORFik-Ex.timings

nameusersystemelapsed
DEG.analysis13.037 0.80416.033
DEG.plot.static0.8330.0581.235
DEG_model0.8930.0611.197
DEG_model_results0.8560.0701.257
DEG_model_simple0.8400.0431.044
DTEG.analysis0.8250.0600.913
DTEG.plot0.8110.0511.005
ORFik.template.experiment0.7730.1021.053
ORFik.template.experiment.zf0.1370.0060.227
ORFikQC0.5450.0350.699
QCreport0.5710.0390.613
QCstats0.5670.0340.603
QCstats.plot0.3640.0340.398
RiboQC.plot0.3470.0520.398
STAR.align.folder000
STAR.align.single0.0010.0010.001
STAR.allsteps.multiQC16.877 0.94421.734
STAR.index000
STAR.install0.0010.0000.000
STAR.multiQC3.7170.1293.914
STAR.remove.crashed.genome0.0000.0010.001
TOP.Motif.ecdf0.0010.0000.001
append_gene_symbols0.7810.0260.808
artificial.orfs0.3000.0010.301
asTX0.4920.0100.490
assignTSSByCage0.0000.0010.003
bamVarName0.3100.0020.312
browseSRA000
canonical_isoforms-experiment-method0.8360.0380.875
canonical_isoforms0.8190.0400.859
codon_usage4.5590.2184.736
codon_usage_exp16.120 1.79618.498
codon_usage_plot5.5260.9257.046
collapse.by.scores0.2700.0670.549
collapse.fastq0.0630.0090.146
collapseDuplicatedReads-GAlignmentPairs-method0.1330.0110.178
collapseDuplicatedReads-GAlignments-method0.0510.0040.083
collapseDuplicatedReads-GRanges-method0.0490.0020.051
collapseDuplicatedReads-data.table-method0.0570.0020.102
collapseDuplicatedReads0.0600.0040.082
combn.pairs0.6570.0360.968
computeFeatures8.0640.7939.197
computeFeaturesCage000
config0.0000.0010.000
config.exper0.2980.0360.871
config.save000
config_file0.4030.0310.868
convertLibs0.6790.0431.830
convertToOneBasedRanges0.2690.0340.606
convert_bam_to_ofst0.7450.0661.442
convert_to_bigWig1.9960.0853.725
convert_to_covRle1.1120.0391.238
convert_to_covRleList1.2670.0651.329
countOverlapsW0.0710.0030.074
countTable0.3300.0390.371
countTable_regions4.1451.6062.896
covRle0.9740.4510.060
covRleFromGR0.0980.0120.110
covRleList0.0180.0040.022
coverageByTranscriptFST0.1560.0190.175
coverageHeatMap1.4160.1451.535
coveragePerTiling0.7790.0700.849
coverageScorings0.0170.0020.012
create.experiment0.7850.0280.830
defineTrailer0.1420.0100.152
design-experiment-method0.3200.0120.332
detectRibosomeShifts0.0020.0010.003
detect_drive0.0010.0020.005
detect_ribo_orfs5.4040.5745.524
disengagementScore0.3870.0220.409
distToCds0.1440.0090.153
distToTSS0.1310.0080.139
download.SRA000
download.SRA.metadata0.2580.0700.589
download_gene_info0.1700.0050.229
entropy1.3940.0561.408
exonsWithPseudoIntronsPerGroup1.6790.1621.797
experiment-class0.3720.0110.382
export.bed120.0180.0010.018
export.bigWig0.0340.0040.038
export.fstwig0.0530.0030.055
export.ofst-GAlignmentPairs-method0.0930.0060.100
export.ofst-GAlignments-method0.0890.0030.093
export.ofst-GRanges-method0.0890.0010.090
export.ofst-data.frame-method0.0920.0020.095
export.ofst0.0880.0000.088
export.wiggle0.0290.0010.030
extendLeaders0.8260.0340.862
extendLeadersUntil1.0550.0210.982
extendTrailers0.5680.0470.609
extendTrailersUntil1.0970.0501.053
extract_run_id0.0000.0000.001
file_ext_without_compression0.0000.0010.001
filepath0.3730.0060.379
filterTranscripts1.2160.0361.253
fimport0.3080.0150.323
findFa0.0040.0000.004
findMapORFs0.3740.0010.374
findORFs0.1450.0020.147
findORFsFasta0.0660.0020.061
findPeaksPerGene0.9590.0100.958
findUORFs0.0020.0000.001
findUORFs_exp3.8660.0513.918
find_url_ebi0.1620.0141.298
firstEndPerGroup0.0990.0010.100
firstExonPerGroup0.0900.0310.121
firstStartPerGroup0.0540.0260.080
fix_malformed_gff0.0000.0000.001
flankPerGroup0.0770.0340.106
floss0.3080.1270.410
fpkm0.1370.0160.118
fractionLength0.0610.0050.054
fread.bed0.0290.0080.037
gcContent0.2980.0400.338
geneToSymbol000
getGenomeAndAnnotation0.0010.0000.000
get_bioproject_candidates000
get_genome_fasta0.0010.0000.000
get_genome_gtf0.0010.0000.000
get_noncoding_rna0.0000.0010.000
get_phix_genome0.0010.0000.001
get_silva_rRNA0.0010.0000.000
get_system_usage0.1190.0960.405
go_analaysis_gorilla0.0030.0010.003
groupGRangesBy0.0920.0280.121
groupings0.0400.0020.042
heatMapRegion0.3050.0280.334
import.ofst0.0630.0010.065
initiationScore0.8380.2061.036
insideOutsideORF0.6660.1080.751
install.fastp000
install.sratoolkit000
isInFrame0.1520.0080.161
isOverlapping0.1640.0070.172
kozakHeatmap0.0010.0000.000
kozakSequenceScore0.4220.0220.429
lastExonEndPerGroup0.0590.0020.061
lastExonPerGroup0.0970.0160.113
lastExonStartPerGroup0.0480.0010.049
libraryTypes0.2910.0020.294
list.experiments0.2880.0330.321
list.genomes0.0000.0020.002
loadRegion1.3070.1091.418
loadRegions0.5690.0320.601
loadTranscriptType000
loadTxdb0.1420.0040.147
longestORFs0.0980.0030.095
makeGRangesFromDataFrameFast6.0440.4886.532
makeGRangesListFromCharacter0.0670.0000.068
makeORFNames0.0580.0000.058
makeSummarizedExperimentFromBam0.2970.0090.306
makeTxdbFromGenome24.528 1.00126.314
mergeFastq0.1070.0110.119
mergeLibs0.7360.2760.887
metaWindow0.7630.0220.789
model.matrix-experiment-method0.4870.0190.509
numExonsPerGroup0.0370.0000.037
optimizedTranscriptLengths1.3470.0321.379
orfFrameDistributions68.17810.64130.505
orfScore27.431 3.555 3.526
organism-experiment-method0.6910.0670.760
outputLibs2.1632.7424.578
pSitePlot1.7080.3042.241
pcaExperiment6.4631.4017.863
pcaPlot1.6920.1121.948
pmapFromTranscriptF0.0730.0020.074
pmapToTranscriptF0.5990.0700.662
pseudoIntronsPerGroup1.3560.2131.551
rankOrder0.0500.0040.054
read.experiment0.2800.0300.309
readBam0.0960.0120.107
readBamIsUniqueMapper0.0890.0170.107
readBamSeqs0.0700.0090.079
readWidths0.0140.0010.015
read_RDSQS0.2970.0310.327
reassignTSSbyCage0.4740.0490.522
reassignTxDbByCage000
reduceKeepAttr0.0860.0100.096
regionPerReadLength0.2730.2870.892
remove.experiments0.6580.4130.629
riboORFs0.3310.0380.370
riboORFsFolder0.6310.1340.766
ribo_fft4.5890.5356.096
ribo_fft_plot4.8810.3186.185
ribosomeReleaseScore0.1710.0230.188
ribosomeStallingScore0.2100.0170.220
save.experiment0.3020.0010.303
save_RDSQS0.0060.0020.004
scaledWindowPositions0.4110.0300.438
seqnamesPerGroup0.0520.0070.057
shiftFootprints0.0030.0000.002
shiftFootprintsByExperiment2.0790.1948.001
shiftPlots4.5572.0920.758
shifts_load0.6620.0730.735
shifts_save0.0910.0100.100
simpleLibs0.5560.0510.608
sortPerGroup0.4320.0560.706
startCodons0.8850.0941.130
startDefinition0.0010.0000.001
startRegion0.7590.0680.827
startRegionCoverage1.1350.2141.505
startSites0.4810.0450.688
stopCodons0.8720.0751.150
stopDefinition0.0010.0000.001
stopRegion0.7280.0900.819
stopSites0.3730.1200.493
strandBool0.0130.0070.020
strandPerGroup0.0710.0140.085
subsetToFrame0.0270.0050.032
symbols0.5930.0930.737
te.plot0.0000.0000.001
te.table0.5520.1310.683
te_rna.plot0.4740.0370.512
template_shift_table0.3610.0440.405
tile10.1400.0130.153
topMotif000
transcriptWindow1.1040.3631.626
translationalEff0.1280.0240.146
trimming.table0.0240.0020.027
txNames0.0310.0040.035
txNamesToGeneNames0.9510.0791.035
uORFSearchSpace0.6050.0270.633
uniqueGroups0.2480.0210.269
uniqueOrder0.3820.0280.410
unlistGrl0.1120.0110.124
widthPerGroup0.1110.0050.117
windowCoveragePlot2.5130.1523.399
windowPerGroup1.5990.0612.364
windowPerReadLength1.4110.0741.626