Back to Build/check report for BioC 3.24:   simplified   long
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This page was generated on 2026-05-02 11:33 -0400 (Sat, 02 May 2026).

HostnameOSArch (*)R versionInstalled pkgs
nebbiolo2Linux (Ubuntu 24.04.4 LTS)x86_644.6.0 RC (2026-04-17 r89917) -- "Because it was There" 4844
Click on any hostname to see more info about the system (e.g. compilers)      (*) as reported by 'uname -p', except on Windows and Mac OS X

Package 2034/2366HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
singleCellTK 2.23.0  (landing page)
Joshua David Campbell
Snapshot Date: 2026-05-01 13:45 -0400 (Fri, 01 May 2026)
git_url: https://git.bioconductor.org/packages/singleCellTK
git_branch: devel
git_last_commit: 3ac722f
git_last_commit_date: 2026-04-28 09:03:00 -0400 (Tue, 28 Apr 2026)
nebbiolo2Linux (Ubuntu 24.04.4 LTS) / x86_64  OK    OK    WARNINGS  UNNEEDED, same version is already published
See other builds for singleCellTK in R Universe.


CHECK results for singleCellTK on nebbiolo2

To the developers/maintainers of the singleCellTK package:
- Allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/singleCellTK.git to reflect on this report. See Troubleshooting Build Report for more information.
- Use the following Renviron settings to reproduce errors and warnings.
- If 'R CMD check' started to fail recently on the Linux builder(s) over a missing dependency, add the missing dependency to 'Suggests:' in your DESCRIPTION file. See Renviron.bioc for more information.

raw results


Summary

Package: singleCellTK
Version: 2.23.0
Command: /home/biocbuild/bbs-3.24-bioc/R/bin/R CMD check --install=check:singleCellTK.install-out.txt --library=/home/biocbuild/bbs-3.24-bioc/R/site-library --timings singleCellTK_2.23.0.tar.gz
StartedAt: 2026-05-02 04:37:41 -0400 (Sat, 02 May 2026)
EndedAt: 2026-05-02 04:53:59 -0400 (Sat, 02 May 2026)
EllapsedTime: 977.9 seconds
RetCode: 0
Status:   WARNINGS  
CheckDir: singleCellTK.Rcheck
Warnings: 3

Command output

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.24-bioc/R/bin/R CMD check --install=check:singleCellTK.install-out.txt --library=/home/biocbuild/bbs-3.24-bioc/R/site-library --timings singleCellTK_2.23.0.tar.gz
###
##############################################################################
##############################################################################


* using log directory ‘/home/biocbuild/bbs-3.24-bioc/meat/singleCellTK.Rcheck’
* using R version 4.6.0 RC (2026-04-17 r89917)
* using platform: x86_64-pc-linux-gnu
* R was compiled by
    gcc (Ubuntu 13.3.0-6ubuntu2~24.04.1) 13.3.0
    GNU Fortran (Ubuntu 13.3.0-6ubuntu2~24.04.1) 13.3.0
* running under: Ubuntu 24.04.4 LTS
* using session charset: UTF-8
* current time: 2026-05-02 08:37:41 UTC
* checking for file ‘singleCellTK/DESCRIPTION’ ... OK
* checking extension type ... Package
* this is package ‘singleCellTK’ version ‘2.23.0’
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... INFO
Imports includes 80 non-default packages.
Importing from so many packages makes the package vulnerable to any of
them becoming unavailable.  Move as many as possible to Suggests and
use conditionally.
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘singleCellTK’ can be installed ... OK
* checking installed package size ... INFO
  installed size is  7.0Mb
  sub-directories of 1Mb or more:
    R         1.0Mb
    extdata   1.6Mb
    shiny     3.0Mb
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking code files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking loading without being on the library search path ... OK
* checking whether startup messages can be suppressed ... OK
* checking dependencies in R code ... WARNING
Missing or unexported object: 'harmony::HarmonyMatrix'
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... WARNING
Missing link(s) in Rd file 'runHarmony.Rd':
  ‘[harmony]{HarmonyMatrix}’

See section 'Cross-references' in the 'Writing R Extensions' manual.

Found the following Rd file(s) with Rd \link{} targets missing package
anchors:
  dedupRowNames.Rd: SingleCellExperiment-class
  detectCellOutlier.Rd: colData
  diffAbundanceFET.Rd: colData
  downSampleCells.Rd: SingleCellExperiment-class
  downSampleDepth.Rd: SingleCellExperiment-class
  featureIndex.Rd: SummarizedExperiment-class,
    SingleCellExperiment-class
  getBiomarker.Rd: SingleCellExperiment-class
  getDEGTopTable.Rd: SingleCellExperiment-class
  getEnrichRResult.Rd: SingleCellExperiment-class
  getFindMarkerTopTable.Rd: SingleCellExperiment-class
  getGenesetNamesFromCollection.Rd: SingleCellExperiment-class
  getPathwayResultNames.Rd: SingleCellExperiment-class
  getSampleSummaryStatsTable.Rd: SingleCellExperiment-class, assay,
    colData
  getSoupX.Rd: SingleCellExperiment-class
  getTSCANResults.Rd: SingleCellExperiment-class
  getTopHVG.Rd: SingleCellExperiment-class
  importAlevin.Rd: DelayedArray, readMM
  importAnnData.Rd: DelayedArray, readMM
  importBUStools.Rd: readMM
  importCellRanger.Rd: readMM, DelayedArray
  importCellRangerV2Sample.Rd: readMM, DelayedArray
  importCellRangerV3Sample.Rd: readMM, DelayedArray
  importDropEst.Rd: DelayedArray, readMM
  importExampleData.Rd: scRNAseq, Matrix, DelayedArray,
    ReprocessedFluidigmData, ReprocessedAllenData, NestorowaHSCData
  importFromFiles.Rd: readMM, DelayedArray, SingleCellExperiment-class
  importGeneSetsFromCollection.Rd: GeneSetCollection-class,
    SingleCellExperiment-class, GeneSetCollection, GSEABase, metadata
  importGeneSetsFromGMT.Rd: GeneSetCollection-class,
    SingleCellExperiment-class, getGmt, GSEABase, metadata
  importGeneSetsFromList.Rd: GeneSetCollection-class,
    SingleCellExperiment-class, GSEABase, metadata
  importGeneSetsFromMSigDB.Rd: SingleCellExperiment-class, msigdbr,
    GeneSetCollection-class, GSEABase, metadata
  importMitoGeneSet.Rd: SingleCellExperiment-class,
    GeneSetCollection-class, GSEABase, metadata
  importMultipleSources.Rd: DelayedArray
  importOptimus.Rd: readMM, DelayedArray
  importSEQC.Rd: readMM, DelayedArray
  importSTARsolo.Rd: readMM, DelayedArray
  iterateSimulations.Rd: SingleCellExperiment-class
  listSampleSummaryStatsTables.Rd: SingleCellExperiment-class, metadata
  plotBarcodeRankDropsResults.Rd: SingleCellExperiment-class
  plotBarcodeRankScatter.Rd: SingleCellExperiment-class
  plotBatchCorrCompare.Rd: SingleCellExperiment-class
  plotBatchVariance.Rd: SingleCellExperiment-class
  plotBcdsResults.Rd: SingleCellExperiment-class
  plotClusterAbundance.Rd: colData
  plotCxdsResults.Rd: SingleCellExperiment-class
  plotDEGHeatmap.Rd: SingleCellExperiment-class
  plotDEGRegression.Rd: SingleCellExperiment-class
  plotDEGViolin.Rd: SingleCellExperiment-class
  plotDEGVolcano.Rd: SingleCellExperiment-class
  plotDecontXResults.Rd: SingleCellExperiment-class
  plotDoubletFinderResults.Rd: SingleCellExperiment-class
  plotEmptyDropsResults.Rd: SingleCellExperiment-class
  plotEmptyDropsScatter.Rd: SingleCellExperiment-class
  plotEnrichR.Rd: SingleCellExperiment-class
  plotFindMarkerHeatmap.Rd: SingleCellExperiment-class
  plotPCA.Rd: SingleCellExperiment-class
  plotPathway.Rd: SingleCellExperiment-class
  plotRunPerCellQCResults.Rd: SingleCellExperiment-class
  plotSCEBarAssayData.Rd: SingleCellExperiment-class
  plotSCEBarColData.Rd: SingleCellExperiment-class
  plotSCEBatchFeatureMean.Rd: SingleCellExperiment-class
  plotSCEDensity.Rd: SingleCellExperiment-class
  plotSCEDensityAssayData.Rd: SingleCellExperiment-class
  plotSCEDensityColData.Rd: SingleCellExperiment-class
  plotSCEDimReduceColData.Rd: SingleCellExperiment-class
  plotSCEDimReduceFeatures.Rd: SingleCellExperiment-class
  plotSCEHeatmap.Rd: SingleCellExperiment-class
  plotSCEScatter.Rd: SingleCellExperiment-class
  plotSCEViolin.Rd: SingleCellExperiment-class
  plotSCEViolinAssayData.Rd: SingleCellExperiment-class
  plotSCEViolinColData.Rd: SingleCellExperiment-class
  plotScDblFinderResults.Rd: SingleCellExperiment-class
  plotScdsHybridResults.Rd: SingleCellExperiment-class
  plotScrubletResults.Rd: SingleCellExperiment-class
  plotSoupXResults.Rd: SingleCellExperiment-class
  plotTSCANClusterDEG.Rd: SingleCellExperiment-class
  plotTSCANClusterPseudo.Rd: SingleCellExperiment-class
  plotTSCANDimReduceFeatures.Rd: SingleCellExperiment-class
  plotTSCANPseudotimeGenes.Rd: SingleCellExperiment-class
  plotTSCANPseudotimeHeatmap.Rd: SingleCellExperiment-class
  plotTSCANResults.Rd: SingleCellExperiment-class
  plotTSNE.Rd: SingleCellExperiment-class
  plotUMAP.Rd: SingleCellExperiment-class
  readSingleCellMatrix.Rd: DelayedArray
  reportCellQC.Rd: SingleCellExperiment-class
  reportClusterAbundance.Rd: colData
  reportDiffAbundanceFET.Rd: colData
  retrieveSCEIndex.Rd: SingleCellExperiment-class
  runBBKNN.Rd: SingleCellExperiment-class
  runBarcodeRankDrops.Rd: SingleCellExperiment-class, colData
  runBcds.Rd: SingleCellExperiment-class, colData
  runCellQC.Rd: colData
  runComBatSeq.Rd: SingleCellExperiment-class
  runCxds.Rd: SingleCellExperiment-class, colData
  runCxdsBcdsHybrid.Rd: colData
  runDEAnalysis.Rd: SingleCellExperiment-class
  runDecontX.Rd: colData
  runDimReduce.Rd: SingleCellExperiment-class
  runDoubletFinder.Rd: SingleCellExperiment-class
  runDropletQC.Rd: colData
  runEmptyDrops.Rd: SingleCellExperiment-class, colData
  runEnrichR.Rd: SingleCellExperiment-class
  runFastMNN.Rd: SingleCellExperiment-class, BiocParallelParam-class
  runFeatureSelection.Rd: SingleCellExperiment-class
  runFindMarker.Rd: SingleCellExperiment-class
  runGSVA.Rd: SingleCellExperiment-class
  runHarmony.Rd: SingleCellExperiment-class
  runKMeans.Rd: SingleCellExperiment-class, colData
  runLimmaBC.Rd: SingleCellExperiment-class, assay
  runMNNCorrect.Rd: SingleCellExperiment-class, assay,
    BiocParallelParam-class
  runModelGeneVar.Rd: SingleCellExperiment-class
  runPerCellQC.Rd: SingleCellExperiment-class, BiocParallelParam,
    colData
  runSCANORAMA.Rd: SingleCellExperiment-class, assay
  runSCMerge.Rd: SingleCellExperiment-class, colData, assay,
    BiocParallelParam-class
  runScDblFinder.Rd: SingleCellExperiment-class, colData
  runScranSNN.Rd: SingleCellExperiment-class, reducedDim, assay,
    altExp, colData, igraph
  runScrublet.Rd: SingleCellExperiment-class, colData
  runSingleR.Rd: SingleCellExperiment-class
  runSoupX.Rd: SingleCellExperiment-class
  runTSCAN.Rd: SingleCellExperiment-class
  runTSCANClusterDEAnalysis.Rd: SingleCellExperiment-class
  runTSCANDEG.Rd: SingleCellExperiment-class
  runTSNE.Rd: SingleCellExperiment-class
  runUMAP.Rd: SingleCellExperiment-class, BiocParallelParam-class
  runVAM.Rd: SingleCellExperiment-class
  runZINBWaVE.Rd: SingleCellExperiment-class, colData,
    BiocParallelParam-class
  sampleSummaryStats.Rd: SingleCellExperiment-class, assay, colData
  scaterPCA.Rd: SingleCellExperiment-class, BiocParallelParam-class
  scaterlogNormCounts.Rd: logNormCounts
  sctkListGeneSetCollections.Rd: GeneSetCollection-class
  sctkPythonInstallConda.Rd: conda_install, reticulate, conda_create
  sctkPythonInstallVirtualEnv.Rd: virtualenv_install, reticulate,
    virtualenv_create
  selectSCTKConda.Rd: reticulate
  selectSCTKVirtualEnvironment.Rd: reticulate
  setRowNames.Rd: SingleCellExperiment-class
  setSCTKDisplayRow.Rd: SingleCellExperiment-class
  singleCellTK.Rd: SingleCellExperiment-class
  subsetSCECols.Rd: SingleCellExperiment-class
  subsetSCERows.Rd: SingleCellExperiment-class, altExp
  summarizeSCE.Rd: SingleCellExperiment-class
Please provide package anchors for all Rd \link{} targets not in the
package itself and the base packages.
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking contents of ‘data’ directory ... OK
* checking data for non-ASCII characters ... OK
* checking data for ASCII and uncompressed saves ... OK
* checking R/sysdata.rda ... OK
* checking files in ‘vignettes’ ... OK
* checking examples ... WARNING
Found the following significant warnings:

  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'librarySizeFactors' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in fitTrendVar(fm, fv, ...) : 'fitTrendVar' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in fitTrendVar(fm, fv, ...) : 'fitTrendVar' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'sumCountsAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'summarizeAssayByGroup' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in fitTrendVar(fm, fv, ...) : 'fitTrendVar' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'librarySizeFactors' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in fitTrendVar(fm, fv, ...) : 'fitTrendVar' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
Deprecated functions may be defunct as soon as of the next release of
R.
See ?Deprecated.
Examples with CPU (user + system) or elapsed time > 5s
                           user system elapsed
plotDoubletFinderResults 35.757  0.236  35.993
runSeuratSCTransform     31.049  1.149  32.205
runDoubletFinder         31.925  0.213  32.138
plotScDblFinderResults   28.317  0.458  25.840
runScDblFinder           19.276  0.123  17.489
plotBatchCorrCompare     12.240  0.123  12.364
plotScdsHybridResults    10.583  0.020  10.000
importExampleData        10.031  0.405  10.876
plotBcdsResults           8.811  0.211   8.394
plotDecontXResults        8.280  0.146   8.426
runUMAP                   7.349  0.347   7.696
plotCxdsResults           7.079  0.079   7.157
detectCellOutlier         6.748  0.173   6.923
runDecontX                6.700  0.112   6.812
plotEmptyDropsResults     6.651  0.001   6.652
plotEmptyDropsScatter     6.630  0.015   6.645
plotUMAP                  6.558  0.056   6.614
runEmptyDrops             6.316  0.006   6.322
plotDEGViolin             5.384  0.069   5.448
convertSCEToSeurat        4.760  0.291   5.050
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘spelling.R’
  Running ‘testthat.R’
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes ... OK
* checking re-building of vignette outputs ... OK
* checking PDF version of manual ... OK
* DONE

Status: 3 WARNINGs
See
  ‘/home/biocbuild/bbs-3.24-bioc/meat/singleCellTK.Rcheck/00check.log’
for details.


Installation output

singleCellTK.Rcheck/00install.out

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.24-bioc/R/bin/R CMD INSTALL singleCellTK
###
##############################################################################
##############################################################################


* installing to library ‘/home/biocbuild/bbs-3.24-bioc/R/site-library’
* installing *source* package ‘singleCellTK’ ...
** this is package ‘singleCellTK’ version ‘2.23.0’
** using staged installation
** R
** data
** exec
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (singleCellTK)

Tests output

singleCellTK.Rcheck/tests/spelling.Rout


R version 4.6.0 RC (2026-04-17 r89917) -- "Because it was There"
Copyright (C) 2026 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> if (requireNamespace('spelling', quietly = TRUE))
+   spelling::spell_check_test(vignettes = TRUE, error = FALSE, skip_on_cran = TRUE)
All Done!
> 
> proc.time()
   user  system elapsed 
  0.145   0.046   0.177 

singleCellTK.Rcheck/tests/testthat.Rout


R version 4.6.0 RC (2026-04-17 r89917) -- "Because it was There"
Copyright (C) 2026 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(testthat)
> library(singleCellTK)
Loading required package: SummarizedExperiment
Loading required package: MatrixGenerics
Loading required package: matrixStats

Attaching package: 'MatrixGenerics'

The following objects are masked from 'package:matrixStats':

    colAlls, colAnyNAs, colAnys, colAvgsPerRowSet, colCollapse,
    colCounts, colCummaxs, colCummins, colCumprods, colCumsums,
    colDiffs, colIQRDiffs, colIQRs, colLogSumExps, colMadDiffs,
    colMads, colMaxs, colMeans2, colMedians, colMins, colOrderStats,
    colProds, colQuantiles, colRanges, colRanks, colSdDiffs, colSds,
    colSums2, colTabulates, colVarDiffs, colVars, colWeightedMads,
    colWeightedMeans, colWeightedMedians, colWeightedSds,
    colWeightedVars, rowAlls, rowAnyNAs, rowAnys, rowAvgsPerColSet,
    rowCollapse, rowCounts, rowCummaxs, rowCummins, rowCumprods,
    rowCumsums, rowDiffs, rowIQRDiffs, rowIQRs, rowLogSumExps,
    rowMadDiffs, rowMads, rowMaxs, rowMeans2, rowMedians, rowMins,
    rowOrderStats, rowProds, rowQuantiles, rowRanges, rowRanks,
    rowSdDiffs, rowSds, rowSums2, rowTabulates, rowVarDiffs, rowVars,
    rowWeightedMads, rowWeightedMeans, rowWeightedMedians,
    rowWeightedSds, rowWeightedVars

Loading required package: GenomicRanges
Loading required package: stats4
Loading required package: BiocGenerics
Loading required package: generics

Attaching package: 'generics'

The following objects are masked from 'package:base':

    as.difftime, as.factor, as.ordered, intersect, is.element, setdiff,
    setequal, union


Attaching package: 'BiocGenerics'

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, is.unsorted, lapply,
    mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int,
    rank, rbind, rownames, sapply, saveRDS, table, tapply, unique,
    unsplit, which.max, which.min

Loading required package: S4Vectors

Attaching package: 'S4Vectors'

The following object is masked from 'package:utils':

    findMatches

The following objects are masked from 'package:base':

    I, expand.grid, unname

Loading required package: IRanges
Loading required package: Seqinfo
Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
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Attaching package: 'Biobase'

The following object is masked from 'package:MatrixGenerics':

    rowMedians

The following objects are masked from 'package:matrixStats':

    anyMissing, rowMedians

Loading required package: SingleCellExperiment
Loading required package: DelayedArray
Loading required package: Matrix

Attaching package: 'Matrix'

The following object is masked from 'package:S4Vectors':

    expand

Loading required package: S4Arrays
Loading required package: abind

Attaching package: 'S4Arrays'

The following object is masked from 'package:abind':

    abind

The following object is masked from 'package:base':

    rowsum

Loading required package: SparseArray

Attaching package: 'DelayedArray'

The following objects are masked from 'package:base':

    apply, scale, sweep


Attaching package: 'singleCellTK'

The following object is masked from 'package:BiocGenerics':

    plotPCA

> 
> test_check("singleCellTK")
Found 2 batches
Using null model in ComBat-seq.
Adjusting for 0 covariate(s) or covariate level(s)
Estimating dispersions
Fitting the GLM model
Shrinkage off - using GLM estimates for parameters
Adjusting the data
Found 2 batches
Using null model in ComBat-seq.
Adjusting for 1 covariate(s) or covariate level(s)
Estimating dispersions
Fitting the GLM model
Shrinkage off - using GLM estimates for parameters
Adjusting the data
Performing log-normalization
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Calculating gene variances
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
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Calculating gene variances
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Uploading data to Enrichr... Done.
  Querying HDSigDB_Human_2021... Done.
Parsing results... Done.
Performing log-normalization
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variances
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene means
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variance to mean ratios
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene means
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variance to mean ratios
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Performing log-normalization
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variances
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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[04:51:43] WARNING: src/learner.cc:782: 
Parameters: { "nthreads" } are not used.

[04:51:44] WARNING: src/learner.cc:782: 
Parameters: { "nthreads" } are not used.

Performing log-normalization
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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Calculating gene variances
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck

Number of nodes: 390
Number of edges: 9849

Running Louvain algorithm...
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Maximum modularity in 10 random starts: 0.8351
Number of communities: 7
Elapsed time: 0 seconds
Using method 'umap'
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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Performing log-normalization
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
[ FAIL 0 | WARN 84 | SKIP 0 | PASS 225 ]

[ FAIL 0 | WARN 84 | SKIP 0 | PASS 225 ]
> 
> proc.time()
   user  system elapsed 
282.746   7.032 291.208 

Example timings

singleCellTK.Rcheck/singleCellTK-Ex.timings

nameusersystemelapsed
MitoGenes0.0020.0000.003
SEG0.0020.0000.003
calcEffectSizes0.1510.0050.158
combineSCE0.7820.1010.883
computeZScore0.2910.0160.306
convertSCEToSeurat4.7600.2915.050
convertSeuratToSCE0.3360.0010.337
dedupRowNames0.0640.0000.064
detectCellOutlier6.7480.1736.923
diffAbundanceFET0.0580.0020.060
discreteColorPalette0.0080.0000.008
distinctColors0.0030.0000.003
downSampleCells0.5650.0330.598
downSampleDepth0.4580.0110.468
expData-ANY-character-method0.1250.0020.127
expData-set-ANY-character-CharacterOrNullOrMissing-logical-method0.1650.0000.165
expData-set0.1550.0000.155
expData0.1270.0010.129
expDataNames-ANY-method0.120.000.12
expDataNames0.1180.0020.120
expDeleteDataTag0.0320.0000.032
expSetDataTag0.0230.0000.023
expTaggedData0.0240.0000.024
exportSCE0.0200.0010.021
exportSCEtoAnnData0.0950.0020.097
exportSCEtoFlatFile0.0880.0070.095
featureIndex0.0360.0010.037
generateSimulatedData0.0520.0000.053
getBiomarker0.0620.0000.062
getDEGTopTable0.6970.0660.763
getDiffAbundanceResults0.050.000.05
getEnrichRResult0.6000.0323.841
getFindMarkerTopTable1.6130.0221.635
getMSigDBTable0.0030.0000.003
getPathwayResultNames0.0210.0010.022
getSampleSummaryStatsTable0.2690.0010.270
getSoupX000
getTSCANResults1.0900.0191.109
getTopHVG0.7700.0050.776
importAnnData0.0010.0000.001
importBUStools0.1550.0010.156
importCellRanger0.7990.0200.820
importCellRangerV2Sample0.1510.0050.156
importCellRangerV3Sample0.2920.0080.301
importDropEst0.1950.0010.196
importExampleData10.031 0.40510.876
importGeneSetsFromCollection0.0730.0000.073
importGeneSetsFromGMT0.0610.0000.062
importGeneSetsFromList0.1210.0010.122
importGeneSetsFromMSigDB2.0910.0092.100
importMitoGeneSet0.0500.0010.050
importOptimus0.0020.0000.002
importSEQC0.1040.0000.104
importSTARsolo0.1340.0010.135
iterateSimulations0.1620.0010.162
listSampleSummaryStatsTables0.2770.0020.279
mergeSCEColData0.3260.0050.331
mouseBrainSubsetSCE0.0350.0010.035
msigdb_table0.0020.0000.001
plotBarcodeRankDropsResults0.8650.0070.871
plotBarcodeRankScatter0.7630.0020.765
plotBatchCorrCompare12.240 0.12312.364
plotBatchVariance0.4380.0870.525
plotBcdsResults8.8110.2118.394
plotBubble0.7840.0030.787
plotClusterAbundance1.3040.0031.307
plotCxdsResults7.0790.0797.157
plotDEGHeatmap2.1000.0192.119
plotDEGRegression4.2270.0264.247
plotDEGViolin5.3840.0695.448
plotDEGVolcano0.9880.0050.993
plotDecontXResults8.2800.1468.426
plotDimRed0.2750.0010.276
plotDoubletFinderResults35.757 0.23635.993
plotEmptyDropsResults6.6510.0016.652
plotEmptyDropsScatter6.6300.0156.645
plotFindMarkerHeatmap3.7110.0103.720
plotMASTThresholdGenes1.1910.0061.197
plotPCA0.3540.0030.356
plotPathway0.6170.0030.621
plotRunPerCellQCResults2.8530.0152.869
plotSCEBarAssayData0.2630.0000.263
plotSCEBarColData0.2210.0040.225
plotSCEBatchFeatureMean0.3940.0000.395
plotSCEDensity0.3120.0010.312
plotSCEDensityAssayData0.2850.0010.286
plotSCEDensityColData0.2940.0010.295
plotSCEDimReduceColData0.7320.0000.731
plotSCEDimReduceFeatures0.3780.0020.380
plotSCEHeatmap0.4220.0010.424
plotSCEScatter0.3280.0010.328
plotSCEViolin0.3400.0010.341
plotSCEViolinAssayData0.3640.0020.366
plotSCEViolinColData0.3660.0020.367
plotScDblFinderResults28.317 0.45825.840
plotScanpyDotPlot0.0210.0000.022
plotScanpyEmbedding0.0220.0000.021
plotScanpyHVG0.0210.0000.021
plotScanpyHeatmap0.0210.0000.021
plotScanpyMarkerGenes0.0190.0020.021
plotScanpyMarkerGenesDotPlot0.0210.0000.021
plotScanpyMarkerGenesHeatmap0.0210.0000.021
plotScanpyMarkerGenesMatrixPlot0.0210.0000.021
plotScanpyMarkerGenesViolin0.0220.0000.022
plotScanpyMatrixPlot0.0210.0000.021
plotScanpyPCA0.0210.0000.021
plotScanpyPCAGeneRanking0.0210.0000.020
plotScanpyPCAVariance0.0210.0000.021
plotScanpyViolin0.0220.0000.022
plotScdsHybridResults10.583 0.02010.000
plotScrubletResults0.0230.0000.023
plotSeuratElbow0.0230.0000.022
plotSeuratHVG0.0220.0000.022
plotSeuratJackStraw0.0210.0000.022
plotSeuratReduction0.0230.0000.022
plotSoupXResults000
plotTSCANClusterDEG4.5540.0044.559
plotTSCANClusterPseudo1.3210.0051.326
plotTSCANDimReduceFeatures1.2910.0021.293
plotTSCANPseudotimeGenes1.5510.0011.553
plotTSCANPseudotimeHeatmap1.2770.0051.282
plotTSCANResults1.2170.0211.237
plotTSNE0.3750.0020.376
plotTopHVG0.6240.0070.631
plotUMAP6.5580.0566.614
readSingleCellMatrix0.0040.0010.005
reportCellQC0.0790.0010.079
reportDropletQC0.0220.0000.022
reportQCTool0.0770.0010.078
retrieveSCEIndex0.0280.0000.028
runBBKNN0.0010.0000.000
runBarcodeRankDrops0.2190.0000.219
runBcds1.4980.0240.948
runCellQC0.0800.0000.081
runClusterSummaryMetrics0.3650.0030.368
runComBatSeq0.4460.0020.447
runCxds0.3030.0000.303
runCxdsBcdsHybrid1.4690.0110.927
runDEAnalysis0.3730.0010.374
runDecontX6.7000.1126.812
runDimReduce0.2730.0010.274
runDoubletFinder31.925 0.21332.138
runDropletQC0.0230.0000.024
runEmptyDrops6.3160.0066.322
runEnrichR0.5380.0262.751
runFastMNN1.6270.0331.661
runFeatureSelection0.2090.0010.210
runFindMarker1.4850.0011.486
runGSVA0.6330.0050.638
runHarmony0.040.000.04
runKMeans0.2060.0010.207
runLimmaBC0.0810.0010.082
runMNNCorrect0.3760.0020.378
runModelGeneVar0.2720.0010.273
runNormalization2.3720.0142.387
runPerCellQC0.3430.0010.344
runSCANORAMA000
runSCMerge0.0040.0000.004
runScDblFinder19.276 0.12317.489
runScanpyFindClusters0.0250.0000.025
runScanpyFindHVG0.0230.0000.023
runScanpyFindMarkers0.0230.0000.024
runScanpyNormalizeData0.1140.0000.114
runScanpyPCA0.0270.0000.028
runScanpyScaleData0.0250.0000.025
runScanpyTSNE0.0250.0000.025
runScanpyUMAP0.0260.0000.026
runScranSNN0.3580.0010.358
runScrublet0.0240.0000.025
runSeuratFindClusters0.0230.0000.023
runSeuratFindHVG0.5390.0000.539
runSeuratHeatmap0.0240.0000.023
runSeuratICA0.0240.0000.023
runSeuratJackStraw0.0230.0000.023
runSeuratNormalizeData0.0220.0000.022
runSeuratPCA0.0220.0000.022
runSeuratSCTransform31.049 1.14932.205
runSeuratScaleData0.0210.0020.024
runSeuratUMAP0.0200.0020.023
runSingleR0.0390.0010.040
runSoupX000
runTSCAN0.6900.0190.709
runTSCANClusterDEAnalysis0.7570.0110.768
runTSCANDEG0.6960.0120.708
runTSNE0.7290.0130.742
runUMAP7.3490.3477.696
runVAM0.3140.0020.317
runZINBWaVE0.0030.0010.005
sampleSummaryStats0.1600.0020.163
scaterCPM0.1510.0110.163
scaterPCA0.4630.0140.477
scaterlogNormCounts0.2420.0170.259
sce0.0220.0000.022
sctkListGeneSetCollections0.0780.0070.085
sctkPythonInstallConda0.0000.0000.001
sctkPythonInstallVirtualEnv000
selectSCTKConda000
selectSCTKVirtualEnvironment000
setRowNames0.0840.0060.091
setSCTKDisplayRow0.4430.0030.447
singleCellTK000
subDiffEx0.3250.0040.328
subsetSCECols0.0830.0000.083
subsetSCERows0.2290.0090.238
summarizeSCE0.0690.0000.069
trimCounts0.2090.0060.215